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GenomicsTopBP1 associated with RNA in HEK293T cells
TopBP1 associated with RNA in HEK293T cells
PRJNA879057 | ENA
ProteomicsDeciphering the interaction between TopBP1 and GINS using crosslinking mass spectrometry
Recombinant TopBP1 and GINS were cross linked using the MS non-cleavable crosslinker BS3. Crosslinked proteins were then subsequently either digested directly using the SP3 strategy or first seperated on SDS-PAGE and then in-gel digested. cross-linked peptides were identified by LC-MS. The informati...
ORGANISM(S): Homo sapiens (Human) 
2024-05-24 | PXD040156 | Pride
Cross linking Gins Topbp1 Bs3 
ProteomicsMDC1 interacts with TOPBP1 to maintain chromosomal stability during mitosis
MDC1 is a large, modular phospho-protein scaffold that mediates recruitment of signaling and repair complexes to sites of DNA double-strand breaks (DSBs). MDC1 is anchored to damaged chromatin through interaction of its C-terminal BRCT-repeat domain with phosphorylated H2AX (γH2AX), where it recruit...
ORGANISM(S): Homo sapiens (Human) 
2019-03-25 | PXD011850 | Pride
Mdc1 topbp1 mitosis 
ProteomicsConfirmation of replication interactome of TopBP1 using proximity-dependent biotinylation MS
Goal of the Experiment is to confirm the replication interactome of TopBP1 using stabile 293 FlpIn cell lines expressing TopBP1 adapter fused to APEX2. Proteins in its close proximity will become biotinylated by APEX2, thereby enabling the pulldown of biotinylated proteins and thus, replication invo...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD040000 | Pride
Topbp1 Proximity-dependent biotinylation 
GenomicsDownstream Target Screening of TopBP1 in differentiated HPV-positive keratinocytes
Downstream Target Screening of TopBP1 in differentiated HPV-positive keratinocytes
PRJNA503141 | ENA
ProteomicsCIP2A-TOPBP1 Interaction in Human Mitotic Cells
We investigate the implications of DNA double strand breaks in the human genome, regarding the progression and treatment of various cancer types. During mitosis, the canonical DNA repair mechanisms are suspended while broken chromosomes get temporarily stabilized through so-called adapter proteins. ...
ORGANISM(S): Homo sapiens (Human) 
2022-07-19 | PXD034100 | Pride
Uzh Human Cancer Pull-down Mitosis Lc-ms/ms 293t Q-exactive Usz Topbp1 Cip2a Uplc Fgcz Nanoacquity 
ProteomicsBipartite GINS binding mode of TopBP1 to activate the replicative helicase - Chromass Data Set
How the replicative Mcm2-7 helicase is activated during replication origin firing remains largely unknown. Our biochemical and structural studies reveal that the helicase activator GINS interacts with TopBP1 using two separate binding surfaces. One involves a stretch of highly conserved amino acids ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-03-26 | PXD040024 | Pride
Xenopus egg extracts Dna replication 
GenomicsTreacle-dependent TOPBP1 condensation regulates the nucleolar DNA damage response.
Treacle-dependent TOPBP1 condensation regulates the nucleolar DNA damage response.
PRJNA1403436 | ENA
GenomicsTOPBP1 Shapes XY Silencing Dynamics during Meiosis to Ensure Male Fertility
TOPBP1 Shapes XY Silencing Dynamics during Meiosis to Ensure Male Fertility
PRJNA972765 | ENA
ProteomicsThe CIP2A-TOPBP1 axis orchestrates mitotic DNA repair
DNA double-strand breaks (DSBs) accumulate in mitosis in response to DNA replication stress or deficiency in breast cancer susceptibility type 1 and 2 (BRCA1/2). This is a critical threat to genome stability as the classical DSB repair pathways non-homologous end joining (NHEJ) and homologous recomb...
ORGANISM(S): Homo sapiens (Human) 
2025-09-19 | PXD068371 | Pride
Cip2a-topbp1 
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