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MetabolomicsMetabolomic profiling of primary mouse proximal tubule cells (mPTCs) derived from 24-week-old CTR and Atg7 PT-KO kidneys
To explore the role of ATG7 in the kidney PT, we crossed mice homozygous for floxed alleles of Atg7 with Ggt1-Cre mice expressing Cre recombinase under the rat Ggt1 (gamma-glutamyltransferase 1) gene promoter. This generated Atg7fl/fl; γGt1cre/cre mice (hereafter referred to as Atg7 PT-KO), in which...
2026-09-30 | MTBLS15857 | MetaboLights
ProteomicsDetermination of pancreatic cyst fluids peptidome and proteome
Determination of pancreatic cyst fluids (PCF) peptidome and proteome in 5 distinct patient groups: malignant (CAs), intraductal papillary mucinous neoplasms (IPMNs), mucinous cystic neoplasms (MCNs), serous cystic neoplasms (SCNs), and pseudocysts (PCs). The PCF <5kDa fraction, referred as the degra...
ORGANISM(S): Homo sapiens (Human) 
2019-01-14 | PXD005248 | Pride
Human Pancreas Cyst fluids Degradome Proteome Lc-ms/ms 
ProteomicsUnique insights in cervicovaginal Lactobacillus iners and L. crispatus proteome changes associated with microbiome dysbiosis
Cervicovaginal lavage (CVL)supernatants were heat inactivated for 30 minutes at 56°C before further processing. Total protein concentrations were determined using the Pierce Coomassie Plus (Bradford) Protein Assay (Thermo Scientific, Rockford, IL, USA). Sample protein content and volume were normali...
ORGANISM(S): Homo sapiens (Human) Lactobacillus iners Lactobacillus crispatus 
2016-03-15 | PXD003176 | Pride
Label free proteomics Cervicovaginal microbiome dysbiosis 
ProteomicsProteomic Analysis of isolated integrin-associated adhesion complexes in HER2-18 CELLS
Peptide analysis by LC-MS/MS was performed using a nanoACQUITY™ (Waters) Ultra Performance Liquid Chromatography (UPLC) column, coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon).
ORGANISM(S): Homo sapiens (Human) 
2024-11-03 | PXD055405 | Pride
Trastuzumab Adhesion Breast cancer Receptor trafficking Itgb6 Erbb2 Her2 
ProteomicsProteomic analysis of isolated integrin-associated adhesion complexes in Trastuzumab -sensitive and -resistant BT474 cells
Peptide analysis by LC-MS/MS was performed using a nanoACQUITY™ (Waters) Ultra Performance Liquid Chromatography (UPLC) column, coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon).
ORGANISM(S): Homo sapiens (Human) 
2024-11-03 | PXD055603 | Pride
Adhesion Breast cancer Trastuzmab Itgb6 Erbb2 Her2 Herceptin 
ProteomicsProteomic Analysis of isolated integrin-associated adhesion complexes in BT474 cells
Peptide analysis by LC-MS/MS was performed using a nanoACQUITY™ (Waters) Ultra Performance Liquid Chromatography (UPLC) column, coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon).
ORGANISM(S): Homo sapiens (Human) 
2024-11-03 | PXD055411 | Pride
Trastuzumab Adhesion Breast cancer Receptor trafficking Erbb2 Her2 
ProteomicsProteogenomics insights into the hemi-biotrophic pathogen, Phytophthora capsici
This study was designed to evaluate the proteome profiles of Phytophthora capsici. We have utilized a proteogenomics pipeline for identification of proteins from P. capsici.
ORGANISM(S): Phytophthora capsici LT1534 
2022-03-02 | PXD003952 | Pride
Nanoacquity uplc Lc-mse Piper nigrum Proteinlynx global server 
ProteomicsTranscriptomics – assisted proteome profiling of black pepper (Piper nigrum L.)
This study was designed to evaluate the proteome profiles of Piper nigrum when challenged with three different biotic stress. We have utilized a transcriptome-assisted protein identification pipeline coupled with a BLAST2GO annotation.
ORGANISM(S): Piper nigrum 
2022-03-02 | PXD004008 | Pride
Nanoacquity uplc Lc-mse Piper nigrum Proteinlynx global server 
ProteomicsLongitudinal Serum Proteomics In a Mouse Model of Lethal Endotoxemia
A total of thirty-two samples mouse serum samples were analyzed in duplicate (64 total injections) using an LC-MS system comprised of a nanoAcquity LC instrument (Waters nanoAcquity UPLC system, Milford, MA) connected to an Orbitrap Fusion Lumos tribrid mass spectrometer (Thermo Fisher Scientific, S...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2019-10-04 | MSV000084416 | MassIVE
ProteomicsNear complete quantitative proteomics for Yeast
In this study, we attempted to achieve to date the most comprehensive coverage of the yeast proteome during log-phase growth and in the K11R mutant ubiquitin yeast strain with a combination of (1) extensive gel molecular weight fractionation and use of either of two digestion enzymes to improve cove...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-08-26 | PXD001928 | Pride
Yeast High coverage Silac Proteome Lc-ms/ms 
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