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This project was designed to identify a difference in the binding affinity of FILIP1 to different FLNc variants. To this end FLNc domain 1-3 and FLNc d18-21 were used as bait for a pull-down analysis.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-25 | PXD017670 | Pride
mir-154 in mouse lung development - pull-down RNA
This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
Pull-down of PTP-tagged EIF4E2 from bloodstream-form Trypanosoma brucei
ACSL4mRNA probe was designed for pull-down experiments in HaCaT lysates
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD052755 | Pride
proteomic identification of pull-down gel slices of PpSerpin-1 isoforms
ORGANISM(S): Pieris rapae 
2023-08-05 | PXD044199 | Pride
RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
We employed chromatin pull-down and deep sequencing to globally identify HetR DNA targets in vivo at 6 hours after fixed-nitrogen deprivation. We identified novel DNA binding targets of tagged HetR-6xHis and defined a consensus HetR binding site from these HetR target sequences. Chromatin pull down ...
ORGANISM(S): Nostoc sp. PCC 7120 
Mapping microRNA-target interactions in rare cell types with Spy3-AGO2 Pull-down
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