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ProteomicsFlnc d18-21/d1-3 pulldown experiments
The actin-binding protein filamin c (FLNc) is a key mediator in the response of skeletal muscle cells to mechanical stress. In addition to its function as a structural scaffold, FLNc acts as a signaling adaptor which is phosphorylated at S2234 in its mechanosensitive domain 20 (d20) through AKT. Her...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD053236 | Pride
Ap-ms Flnc d18-21 interactome C2 cells/myotubes 
ProteomicsPhosphoproteomics Identifies Dual-Site Phosphorylation in an Extended Basophilic Motif Regulating FILIP1-mediated Degradation of filamin-C FLNc d18-21 BioID experiment
FLNc, the muscle-specific isoform of the filamin family, is a multi-adaptor protein, comprising 1 amino-terminal actin-binding (ABD) domain followed by 24 immunoglobulin-like (Ig) domains. While FLNc can form homodimers via the last Ig-like domain and thus function as an actin-crosslinker like the o...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-12 | PXD009159 | Pride
Bira Flnc Bioid Myotube 
ProteomicsFilaminC pull-down experiment from myotube lysates
This project was designed to identify a difference in the binding affinity of FILIP1 to different FLNc variants. To this end FLNc domain 1-3 and FLNc d18-21 were used as bait for a pull-down analysis.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-25 | PXD017670 | Pride
Pull-down Myotube lysate Flnc 
ProteomicsFLNc Calpain GluC SRM Analysis
Human filamin C (FLNc) is a target of the protease calpain at Y2625. To confirm the dependency of calpain cleavage from FLNc phosphorylation at position S2623/S2624 by PKCa, FLNc was overexpressed in HEK293 cells and cells were treated with PMA to stimulated kinase activity, with Gö6976 to inhibit k...
ORGANISM(S): Homo sapiens (Human) 
2017-01-02 | PXD005097 | Pride
Calpain Flnc Srm 
GenomicsRNA-seq analysis of cardiomyocyte-specific Flnc deficiency and AAV-mediated FLNC reconstitution in mouse hearts
RNA-seq analysis of cardiomyocyte-specific Flnc deficiency and AAV-mediated FLNC reconstitution in mouse hearts
PRJNA1480516 | ENA
ProteomicsFLNc S2233 S2236 PRM analysis
Skeletal muscle is known to adapt dynamically to changes in workload by regulatory processes of the phosphatidylinositide 3-kinase (PI3K)/Akt pathway. We performed a global quantitative phosphoproteomics analysis of contracting mouse C2 myotubes treated with insulin growth factor 1 (IGF-1) or LY2940...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-30 | PXD009228 | Pride
Filamin c Akt Pkc Myocytes 
ProteomicsC-terminal FLNc in vivo kinase assay
The Z-disc is a protein-rich structure critically important for myofibril development and integrity. Since a role of the Z-disc for signal integration and transduction was recently suggested, its precise phosphorylation landscape warranted in-depth analysis. We therefore established a site-resolved ...
ORGANISM(S): Mus musculus (Mouse) 
2017-01-02 | PXD004151 | Pride
Flnc pkc gö6976 
TranscriptomicsRNA-seq analysis of cardiomyocyte-specific Flnc deficiency and AAV-mediated FLNC reconstitution in mouse hearts
Loss-of-function variants in filamin-C (FLNC) are a major genetic cause of dilated cardiomyopathy (DCM) and arrhythmogenic cardiomyopathy (ACM). These variants are associated with progressive ventricular dysfunction, malignant ventricular arrhythmias, heart failure, and an increased risk of sudden c...
ORGANISM(S): Mus musculus 
2026-09-14 | GSE335971 | GEO
ProteomicsIn vitro calpainolysis assays - Myofibrillar Z-discs are a protein phosphorylation hot spot with PKC α modulating protein dynamics
In vitro calpainolysis in combination with top down mass spectrometry reveals the calpain-1 cleavage sites in human and murine carboxy terminal regions of FLNc
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-01-02 | PXD003216 | Pride
Calpain Top down ms Flnc 
ProteomicsLarge-scale in vitro kinase assay with FLNc d23-24 and PKC alpha
Large-scale in vitro kinase assay with human and murine FLNc and its site mutants and PKC alpha.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2017-01-02 | PXD003185 | Pride
Lc-ms In vitro kinase assay Flnc Pkc alpha 
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