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MetabolomicsSample Preparation for Integrated Metabolome and Exposome-Wide Association Studies (MWAS/ExWAS)

Exposome- and metabolome-wide association studies rely on advanced instrumentation, typically anchored in liquid chromatography high-resolution mass spectrometry (LC-HRMS) to investigate exposure-effect associations. Sample preparation for biological fluids is a delicate matter since it is essent...

2026-07-20 | MTBLS15100 | MetaboLights
MetabolomicsA conserved metabolic signature associated with response to fast-acting antimalarial agents

Characterizing the mode of action of antimalarial compounds that emerge from high-throughput phenotypic screens is central to understanding how parasite resistance to these drugs can emerge. Here, we have employed untargeted metabolomics to inform on the mechanism of action of antimalarial leads ...

2023-09-27 | MTBLS6580 | MetaboLights
ProteomicsFilter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos
We report a filter aided, single tip (FAST) method for ultrasensitive proteomic sample preparation. To minimize sample loss and contamination, the method reduces the surface area of the filter to ~0.1 mm2, the total volume of reagents to < 10 μL, and the number of sample transfer steps to two. 25,88...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2022-07-12 | PXD015175 | Pride
Single blastomere Sample preparation Ultrasensitive proteomics 
Proteomicsa novel SDS-based sample preparation for proteomics
Sodium dodecyl sulfate (SDS) is widely used in sample preparation for proteomic research. However, SDS is incompatible with liquid chromatography and electrospray ionization (ESI). SDS depletion is therefore required ahead of liquid chromatography coupled with mass spectrometry (LC-MS) analysis. Mos...
ORGANISM(S): Homo Sapiens Mus Musculus 
2021-03-02 | PXD023557 |
MetabolomicsAbsolute quantification of metabolites in tomato fruit extracts by fast 2D NMR (Tomato 500 MHz samples)

Quantitative NMR metabolomics is a powerful tool to have access to valuable information on metabolism. Unfortunately, the quantitative analysis of metabolic samples is often hampered by peak overlap. Two-dimensional (2D) spectroscopy offers a promising alternative and quantitative results can be ...

2015-01-28 | MTBLS131 | MetaboLights
MetabolomicsAbsolute quantification of metabolites in tomato fruit extracts by fast 2D NMR (Tomato 700 MHz samples)

Quantitative NMR metabolomics is a powerful tool to have access to valuable information on metabolism. Unfortunately, the quantitative analysis of metabolic samples is often hampered by peak overlap. Two-dimensional (2D) spectroscopy offers a promising alternative and quantitative results can be ...

2015-11-11 | MTBLS132 | MetaboLights
ProteomicsMassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications
In proteomics, fast, efficient and highly reproducible sample preparation are of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed the web application MassSpecPreppy that operates on the open ...
ORGANISM(S): Saccharomyces Cerevisiae Strain C13-aby.s86 Escherichia Coli K-12 (ncbitaxon:83333) Homo Sapiens (ncbitaxon:9606) 
2023-07-20 | MSV000092489 | MassIVE
MetabolomicsAbsolute quantification of metabolites in tomato fruit extracts by fast 2D NMR (Calibration Standard 500 MHz samples)

Quantitative NMR metabolomics is a powerful tool to have access to valuable information on metabolism. Unfortunately, the quantitative analysis of metabolic samples is often hampered by peak overlap. Two-dimensional (2D) spectroscopy offers a promising alternative and quantitative results can be ...

2015-11-11 | MTBLS133 | MetaboLights
MetabolomicsAbsolute quantification of metabolites in tomato fruit extracts by fast 2D NMR (Calibration Standard 700 MHz samples)

Quantitative NMR metabolomics is a powerful tool to have access to valuable information on metabolism. Unfortunately, the quantitative analysis of metabolic samples is often hampered by peak overlap. Two-dimensional (2D) spectroscopy offers a promising alternative and quantitative results can be ...

2015-11-11 | MTBLS134 | MetaboLights
ProteomicsA fast, high-sensitivity 96-well plate based MICROFASP method for processing low microgram proteomics sample within 1.5 hours
We report a fast, high-sensitivity MICROFASP method that capable of completing sample preparation in less than 1.5 h which improved the throughput over 13 times compared to the previous reports. In this method, protein digestion time was dramatically shortened from 17 h to 20 min in the limited reac...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-05-07 | PXD053720 | Pride
Fast sample preparation  proteomics analysis  high-sensitivity  high-throughput 
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