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ProteomicsHeat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be influenced by cell culture conditions such as the presence of foetal bovine serum (FBS). Although several studies have evaluated the effect of removing FBS-derived EVs by ultracentrifugation (UC), le...
ORGANISM(S): Bos taurus (Bovine) 
2024-01-26 | PXD047505 | Pride
Ev depletion by ultracentrifugation Foetal bovine serum (fbs) Extracellular vesicles Fbs heat-inactivation 
UnknownHeat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles.
Not available
S-EPMC10805629 | biostudies-literature
UnknownNative proteins from Galdieria sulphuraria to replace fetal bovine serum in mammalian cell culture.
Not available
S-EPMC12065734 | biostudies-literature
UnknownThe Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism.
Not available
S-EPMC7939743 | biostudies-literature
TranscriptomicsTranscription profiling of human gastric epithelial progenitors to H. pylori isolates from Swedish patients with chronic atrophic gastritis 1
Helicobacter pylori infection is associated with development of gastric adenocarcinoma in a subset of infected humans, especially those that develop an antecedent condition, chronic atrophic gastritis (ChAG) characterized by loss of acid-producing parietal cells. Studies in a gnotobiotic transgenic ...
ORGANISM(S): Mus musculus 
2009-11-11 | E-GEOD-16390 | biostudies-arrayexpress
UnknownRapid thermal inactivation of aerosolized SARS-CoV-2.
Not available
S-EPMC8757645 | biostudies-literature
TranscriptomicsResponse of gastric epithelial progenitors to H. pylori isolates from Swedish patients with chronic atrophic gastritis 2
Helicobacter pylori infection is associated with development of gastric adenocarcinoma in a subset of infected humans, especially those that develop an antecedent condition, chronic atrophic gastritis (ChAG) characterized by loss of acid-producing parietal cells. Studies in a gnotobiotic transgenic ...
ORGANISM(S): Helicobacter pylori 
2010-06-20 | E-GEOD-16439 | biostudies-arrayexpress
ProteomicsData-dependent and independent acquisition of Salmonella in epithelial cells throughout the infection process
Viewing the scarce amount of protein material coming from the bacterial pathogen in infection models and despite the availability of contemporary, highly sensitive and fast scanning mass spectrometers, the power requirement still not suffices to study the host and pathogen proteomes simultaneously. ...
ORGANISM(S): Homo sapiens (Human) Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 
2023-07-29 | PXD019863 | Pride
Hela cells Data-dependent acquisition (dda) Proteomics Salmonella Data-independent acquisition (dia) Lc-ms/ms 
ProteomicsData-dependent and independent acquisition of Salmonella in epithelial cells throughout the infection process
Viewing the scarce amount of protein material coming from the bacterial pathogen in infection models and despite the availability of contemporary, highly sensitive and fast scanning mass spectrometers, the power requirement still not suffices to study the host and pathogen proteomes simultaneously. ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-29 | PXD018610 | Pride
Hela cells Data-dependent acquisition (dda) Proteomics Salmonella Data-independent acquisition (dia) Lc-ms/ms 
ProteomicsBlocking ERK activity has a selective but critical impact on activating T cells
The mitogen activated kinases ERK1/2 are activated by antigen receptor engagement and control T cell differentiation. We have used mass spectrometry to explore how ERK1/2 control antigen receptor driven cell growth and proteome restructuring in CD8 T cells. Quantitative analysis of >8000 proteins pr...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-23 | PXD023256 | Pride
Immunology Erk1/2 T cells Mapk 
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