We examined ubiquitin-linkages on Nrf1 purified from cell lysates of NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2. The ubiquitin linkage types were quantified using the LC-MS/MS-based absolute quantification method, ubiquitin parallel reaction monitoring (Ub-AQUA/PRM).
To examine whether ARIH1-UBE2L3 is involved in the atypical ubiquitination of Nrf1 by SCFFBS2, we compared the ubiquitin linkage types between three reactions using GP8: E1/UBE2D1/SCFFBS2, the complete reaction including E1/UBE2D1/SCFFBS2/Nedd8 sys/ARIH1/UBE2L3, and the complete reaction in the abse...
Plasmacytoid dendritic cells (pDCs) play a key role in the activation of the autoimmune response in LN. Recently we demonstrated that these cells infiltrate the kidney of patients with LN at tubulointerstitial level. The pDCs are the main producers of IFN-alpha, whose effects on the renal tubule are...
