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TranscriptomicsTranscription profiling of mouse fetal liver feeder layer and endothelial progenitor cells
While others have reported that fetal liver contains a population of endothelial progenitors based on expression of cell surface markers or culture assays, this is the first proof of a CD31+Sca1+ progenitor by demonstrating highly efficient in vivo angiogenesis and a direct connection to the host va...
ORGANISM(S): Mus musculus 
2007-12-15 | E-GEOD-1727 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human embryonic stem cells (hESC) line Amicqui-1 derived and maintained on human amniotic epithelial cells
Conventional conditions to maintain human embryonic stem cells (hESC) imply the use of inactive mouse embryonic fibroblast (iMEF) as a feeder layer. However, it has suggested that the culture of hESC on iMEF could be an artifact that does not correspond to the in vitro counterpart of the human epibl...
ORGANISM(S): Homo sapiens 
2022-05-01 | E-MTAB-10347 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse undifferentiated embryonic stem cells, differentiated nestin-positive cells and fibroblast feeder layer.
Embryonic stem (ES) cells and ES cell-derived progeny characterized by nestin expression (including neural progenitors) were studied (three independent experiments). The mouse ES cell line R1 was cultured on a feeder layer of mouse embryonic fibroblasts (FL). ES cells were differentiated into nestin...
ORGANISM(S): Mus musculus 
2008-06-12 | E-GEOD-2375 | biostudies-arrayexpress
GenomicsGene expression of human corneal epithelial cells grown with irradiated human fibroblasts or irradiated mouse 3T3 feeder layer .
Gene expression of human corneal epithelial cells grown with irradiated human fibroblasts or irradiated mouse 3T3 feeder layer .
PRJNA594946 | ENA
TranscriptomicsComparable mRNA expression of pluripotency-related genes in SNUhES3 cells cultured on autofeeder or xenofeeder system
I developed a new culture system for hES cells; this system does not require supplementation with bFGF to obtain hES cells that are suitable for tissue engineering and regenerative medicine. This culture system employed mesenchymal stem cells derived from hES cells (hESC-MSCs) as autologous human fe...
ORGANISM(S): Homo sapiens 
2011-02-02 | E-GEOD-26993 | biostudies-arrayexpress
TranscriptomicsUndifferentiated mouse embryonic stem cells, differentiated nestin-positive cells and fibroblast feeder layer.
Embryonic stem (ES) cells and ES cell-derived progeny characterized by nestin expression (including neural progenitors) were studied (three independent experiments). The mouse ES cell line R1 was cultured on a feeder layer of mouse embryonic fibroblasts (FL). ES cells were differentiated into nestin...
ORGANISM(S): Mus musculus 
2005-09-17 | GSE2375 | GEO
TranscriptomicsGene expression of human corneal epithelial cells grown with irradiated human fibroblasts or irradiated mouse 3T3 feeder layer .
human corneal epithelial cells were isolated from healthy human donor eyes. Cells were cultured with irradiated 3T3 (i3T3) murine fibroblast feeder cells, irradiated human corneal fibroblasts (iHFL) or without feeder layer
ORGANISM(S): Homo sapiens 
2019-12-12 | GSE141841 | GEO
TranscriptomicsDevelopment of gene expression signatures for TLX1 overexpression in human thymus C34+ T-cell progenitors cultured on an OP9-DLL1 feeder layer
The T-cell leukemia homeobox 1 (TLX1, HOX11) transcription factor is critically involved in the multistep pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL) and often cooperates with NOTCH1 activation during malignant T-cell transformation. However, the exact molecular mechanisms by which t...
ORGANISM(S): Homo sapiens 
2015-08-05 | E-GEOD-62143 | biostudies-arrayexpress
UnknownA biomimetic synthetic feeder layer supports the proliferation and self-renewal of mouse embryonic stem cells.
Not available
S-EPMC4905775 | biostudies-literature
ProteomicsProteomic analysis of 3T3 murine fibroblast used as feeder-layer
3T3 murine embryonic fibroblasts are used as feeder layers to support the proliferative potential of human primary keratinocytes during in vitro expansion for cell and gene therapy applications. To identify signals mediating this function, we analyzed fibroblast protein content using mass spectromet...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-18 | PXD078052 | Pride
Feeder-layer Fibroblast Skin expansion 
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