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TranscriptomicsA map of open chromatin in human pancreatic islets
High-throughput sequencing of genomic regions isolated using FAIRE (Formaldehyde-assisted isolation of regulatory elements) from three purified pancreatic islet samples For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCO...
ORGANISM(S): Homo sapiens 
2010-01-31 | E-GEOD-17616 | biostudies-arrayexpress
ProteomicsIdentification of spidroin proteins in the silk glands of Nephila clavipes spiders
Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
Spider silk  proteomics  liquid chromatography-tandem mass spectrometry  silk gut 
ProteomicsAging induces hepatic oxidative stress and nuclear proteomic remodelling in liver from Wistar ratAging induces hepatic oxidative stress and nuclear proteomic remodelling in liver from Wistar rats
Aging induces oxidative stress and proteome remodeling in the nucleus from liver in Wistar rats
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-17 | PXD027773 | Pride
Oxidative stress nuclear liver wistar rat lc-ms/ms 
TranscriptomicsOpen chromatin in human pancreatic islets (FAIRE-seq) - Appendix to E-GEOD-17616
An appendix to the published Gaulton et al. work (PMID: 20118932; E-GEOD-17616). In the original paper, the authors note that samples 1 and 2 are not as pure as the third sample. This appendix provides FAIRE-Seq data obtained from a purified islet sample to replace the problematic published data. Th...
ORGANISM(S): Homo sapiens 
2014-12-23 | E-MTAB-3199 | biostudies-arrayexpress
GenomicsRegulatory genomics of human embryonic pancreatic progenitors
Active regulatory regions in the human embryonic pancreatic progenitors were profiled by integration of transcription factor and histone modification ChIP-seq datasets. These were obtained from pancreatic progenitor cells derived in vitro from human embryonic stem cells. The purpose of this work was...
ORGANISM(S): Homo sapiens 
2013-11-15 | E-MTAB-1990 | biostudies-arrayexpress
GenomicsPancreatic islet epigenomics reveals enhancer clusters that are enriched in Type 2 diabetes risk variants
We combined ChIP-seq of chromatin marks and key islet transcription factor with RNA-seq in human islets to map cis-regulatory networks in this primary tissue. The output of this project provides a reference map to dissect genetic variants that alter the susceptibility for Type 2 diabetes, and assist...
ORGANISM(S): Homo sapiens 
2014-01-01 | E-MTAB-1919 | biostudies-arrayexpress
TranscriptomicsTranscriptional and epigenetic high throughput sequencing of human and mouse pancreatic islet-cells
The aim of this experiment was to obtain a detailed transcriptional landscape of human and mouse islet-cells, including strand information, as well an epigenetic map of transcriptionally active chromatin.
ORGANISM(S): Homo sapiens 
2012-10-03 | E-MTAB-1294 | biostudies-arrayexpress
ProteomicsQuiXoT+ project: General Statistical framework for quantitative proteomics by stable isotope labeling
Protein identification was carried out by searching against a joint Human + Yeast Swissprot database (Uniprot release 57.3 May 2009, 26885 entries), in order to increase statistical power of peptide identification, which was supplemented with porcine trypsin, and using SEQUEST algorithm (Bioworks 3....
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-05-11 | PXD000325 | Pride
Yeast Stable isotope labeling Statistical analysis Quantitative proteomics 
GenomicsHES1 ChIP-seq in human embryonic stem cell derived pancreas progenitors Day 13.
To identify genome-wide, direct targets of HES1 in human pancreas progenitors, we performed Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) of endogenous HES1 in the HUES4 PDX1GFP/+ reporter cell line on Day 13 of differentiation from both unsorted bulk populations and FACS-sort...
ORGANISM(S): Homo sapiens 
2022-02-27 | E-MTAB-6922 | biostudies-arrayexpress
TranscriptomicsmiRNA-sequencing of human pancreatic islets and enriched beta-cells
Recent advances in the understanding of the genetics of type 2 diabetes (T2D) susceptibility have focused attention on the regulation of transcriptional activity within the pancreatic beta-cell. MicroRNAs (miRNAs) represent an important component of regulatory control, and have proven roles in the d...
ORGANISM(S): Homo sapiens 
2013-06-07 | E-GEOD-47720 | biostudies-arrayexpress
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