Fetal calf serum (fcs)

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Instrument Platform

Illumina NovaSeq 6000 (22)
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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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2015 (83)
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CHEBI ID

CHEBI:16919 (5)
CHEBI:16027 (4)
CHEBI:16704 (4)
CHEBI:16870 (4)
CHEBI:17568 (4)
CHEBI:30769 (4)
CHEBI:15354 (3)
CHEBI:15741 (3)
CHEBI:15891 (3)
CHEBI:16335 (3)
CHEBI:16737 (3)
CHEBI:16977 (3)
CHEBI:17268 (3)
CHEBI:17295 (3)
CHEBI:17368 (3)
CHEBI:18237 (3)
CHEBI:25094 (3)
CHEBI:27266 (3)
CHEBI:7916 (3)
CHEBI:15366 (2)
CHEBI:15428 (2)
CHEBI:15603 (2)
CHEBI:15713 (2)
CHEBI:15846 (2)
CHEBI:16264 (2)
CHEBI:16414 (2)
CHEBI:16695 (2)
CHEBI:16708 (2)
CHEBI:16761 (2)
CHEBI:16811 (2)

Metabolite Name

AMP (3)
Glycerophosphocholine (3)
ADP (2)
Beta-alanine (2)
Choline (2)
Creatine (2)
Creatine (2)
Creatinine (2)
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GMP (2)
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L-Isoleucine (2)
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L-Phenylalanine (2)
L-Tyrosine (2)
L-Valine (2)
L-alanine (2)
Leucine (2)
NAD+ (2)
UTP (2)
Uridine (2)
1-arachidonoyl-GPE (20:4n6)* (1)
1-linoleoyl-GPC (18:2) (1)
1-linoleoyl-GPE (18:2)* (1)
1-oleoyl-GPC (18:1) (1)
1-oleoyl-GPE (18:1) (1)
1-oleoyl-GPG (18:1)* (1)
1-oleoylglycerol (18:1) (1)

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2014 (18)
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2020 (1)
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Study type

Transcription profiling by array (377)
RNA-seq of coding RNA (55)
ChIP-seq (45)
RNA-seq of coding RNA from single cells (23)
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CLIP-seq (1)

Release Date

2015 (81)
2011 (69)
2014 (61)
2013 (57)
2010 (57)
2012 (52)
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2009 (35)
2008 (32)
2021 (25)
2020 (22)
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2018 (11)
2017 (11)
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2005 (7)
2006 (5)
2004 (5)
2003 (1)

Lab affiliation

Div. Clinical Protein Science and Imaging, Dept. of Clinical Sciences and Dept. of Biomedical Engineering, Lund University, Sweden (2)
University of Liverpool (2)
1) Medizinisches Proteom-Center, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany 2) Institute of Virology, University Hospital, University Duisburg-Essen, Hufelandstr. 55, 45120 Essen, Germany (1)
Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, Netherlands (1)
Bioanalytical Mass Spectrometry Group, Max-Planck-Institute for Biophysical Chemistry Department of Clinical Chemistry, University Medical Center Goettingen (1)
Biozentrum, University of Basel (1)
Cambridge Insitute for Medical Resarch, University of Cambridge (1)
Cell and Chemical Biology LUMC The Netherlands (1)
Center for Medical Genetics, Ghent University and University Hospital, Ghent, Belgium (1)
Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany The Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7BN, UK Max Planck Institute for Molecular Genetics, Berlin, Germany (1)
DZNE Munich Neuroproteomics Feodor-Lynen Str. 17 81377 Munich Germany (1)
Department Biologie Professur für Mikrobiologie (Prof. Dr. Burkovski) (1)
Department for Cell Biology BioMedizinisches Centrum - BMC LMU Muenchen, Med. Faculty Großhaderner Straße 9 82152 Planegg-Martinsried (1)
Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Straße 38, 1090, Vienna, Austria (1)
Department of Analytical Chemistry, University of Vienna (1)
Department of Analytical Chemistry, University of Vienna, Austria (1)
Department of Microgravity and Translational Regenerative Medicine, Otto-von-Guericke University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany (1)
Department of Molecular Mechanisms of Disease, University of Zurich, CH-8057 Zurich, Switzerland (1)
Department of Molecular Medicine, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences (1)
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil (1)
Faculty of Biology Technion-Israel Institute of Technology Haifa, Israel (1)
Faculty of Biology, Technion - Israel Institute of Technology Haifa, Israel (1)
Helmholtz-Centre for Environmental Research - UFZ, Department of Molecular Toxicology (1)
Helmholtz-Centre for Environmental Research – UFZ, Department of Molecular Toxicology (1)
IRCCS IST. G. Gaslini (1)
Institut für Biochemie und Molekularbiologie, Universitätsklinikum Bonn, Rheinische Friedrich-Wilhelms- Universität Bonn, Germany (1)
Institute for Biochemistry and Molecular Biology, University of Bonn, 53115 Bonn, Germany (1)
Institute of Neurology (Edinger Institute), University Hospital Frankfurt, Goethe University, Frankfurt, Germany (1)
Izabela Sumara, Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France (1)
LMU Klinikum Medizinische Klinik und Poliklinik I Campus Großhadern | Marchioninistraße 15 | 81377 München https://www.lmu-klinikum.de/ (1)

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H. ducreyi 35000HP grown in the presence or absence of FCS

Haemophilus ducreyi 35000HP was subjected to different in vitro growth conditions that resemble the in vivo host environment (presence of serum), in order to ascertain the overall changes in gene expression. This information might identify genes important for colonization and/or survival inside the...

ORGANISM(S): Haemophilus ducreyi 35000HP

2010-05-18 | E-GEOD-13851 | biostudies-arrayexpress

Deep analysis of FCS used in cell culture experiment

roteome analysis of commercially available foetal calf serum (FCS) for background correction. Commercially available FCS was depleted of high abundant and enriched for low abundant proteins with commercially available equalizer beads (BioRad) to create a background list of potential contaminates.

ORGANISM(S): Bos taurus (Bovine)

2025-11-17 | PXD062778 | Pride

Fetal calf serum (fcs)

Addressing reproducibility challenges in cell culture: the impact of oxylipins contained in fetal calf serum

Reproducibility issues regarding complex in vitro cell culture experiments have been mainly attributed to genetic variations within cell lines. Batch-dependent fetal calf serum variations are well known and also represent relevant influencing factors. However, the molecular constituents mediating su...

ORGANISM(S): Homo sapiens (Human)

2021-06-01 | PXD020617 | Pride

Eicosanoids Macrophages Reproducibility issues Cell metabolism

Transcription profiling of Mesenchymal stem cells (MSC) maintained in FCS vs autologous serum analysed at passage 4

The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs). To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) with gene expression of MSCs cultured in...

ORGANISM(S): Homo sapiens

2004-12-08 | E-MEXP-214 | biostudies-arrayexpress

Transcription profiling of mesenchymal stem cells (MSC) maintained in FCS or autologous serum over time analysed at passage 4 and 10

The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs)over time. To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) for 10 passages with gene expre...

ORGANISM(S): Homo sapiens

2004-12-08 | E-MEXP-215 | biostudies-arrayexpress

Transcription profiling of human primary fibroblasts from 10 anatomic sites expanded in DMEM supplemented with 10% fetal calf serum, penicillin/streptomycin, and glutamine (Tumor as Wound)

Cancer invasion and metastasis have been likened to wound healing gone awry. Despite striking parallels in cellular behavior between cancer progression and wound healing, the molecular relationships between these two processes, and their prognostic implications, are unclear. Here, based on gene exp...

ORGANISM(S): Homo sapiens

2005-10-31 | E-SMDB-2287 | biostudies-arrayexpress

DNA methylation of mesenchymal stromal cells in platelet lysate versus fetal calf serum

Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology is influenced by these additives – hence they may favor outgrowth of specific subpop...

ORGANISM(S): Homo sapiens

2017-08-11 | GSE87797 | GEO

Gene expression of mesenchymal stromal cells in platelet lysate versus fetal calf serum

ORGANISM(S): Homo sapiens

2017-08-11 | GSE87796 | GEO

BATSeq of ESC-FCS/ESC-2i/NSC

Single-cell polyadenylation site quantification 48 single cells each of murine embryonic stem cells maintained in FCS+LIF medium ("ESC"), embryonic stem cells maintained in serum free, 2i containing medium ("2i") and neural stem cells ("NSC") were sequenced by BATSeq, a...

ORGANISM(S): Mus musculus

2015-06-09 | E-GEOD-60768 | biostudies-arrayexpress

mRNA expression profiling of human mesenchymal stromal cell samples grown in vitro under different conditions (foetal calf serum (FCS) based medium versus human platelet lysate (PL) based medium)

Genetic profiling after stem cell culturing. Culturing cells from normal donors, grown in 2 culture media

ORGANISM(S): Homo sapiens

2023-03-01 | E-MTAB-12272 | biostudies-arrayexpress

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