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ProteomicsC. difficile strain 630 flagellin C modifications
In proteomics, the study of post-translational modifications often relies on some type of enrichment strategy. Here, we show that an approach that is commonly used in phosphoproteomics (Immobilised Metal Affinity Chromatography (Fe3+-IMAC)) also enriches for peptides with a unique post-translational...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2022-04-04 | PXD029552 | Pride
C. difficile Phosphoproteomics Flagellin 
ProteomicsGlycoproteomics of Flagellin C9LY14 from Selenomonas sputigena
The Gram-negative, flagellated, anaerobic, crescent-shaped bacterium Selenomonas sputigena is a potential human periodontal pathogen. Information on its virulence factors and underlying pathogenicity mechanisms is scarce. Here we show for the first time that S. sputigena produces a diversely and hea...
ORGANISM(S): Escherichia coli Selenomonas sputigena ATCC 35185 
2018-01-24 | PXD005859 | Pride
Lc-msms Glycoproteomics Flagellin glycosylation Glycomics Periodontitis Selenomonas sputigena 
ProteomicsIdentification of flagellin peptides in N. benthamiana apoplastic fluid
Purified flagellin from Pseudomonas Pta6605 WT was supplemented with N. benthamiana apoplastic fluids from plants transiently expressing only p19 or p19 and bGAL1 (a galactosidase). By performing subsequent LC/MSMS we wanted to identify and quantify the levels of flagellin peptides released by endog...
ORGANISM(S): Nicotiana benthamiana Pseudomonas amygdali pv. tabaci 
2019-04-15 | PXD011823 | Pride
Pseudomonas Pta6605 Beta-galactosidase Flagellin 
ProteomicsIdentification of flagellin proteins from the citrus canker pathogens Xanthomonas citri (strain 306) and Xanthomonas aurantifolii pathotype C (strain ICMP 8435)
In this project, we have extracted the flagellum of the citrus canker pathogens Xanthomonas citri and Xanthomonas aurantifolii pathotype C and the proteins associated with these preparations were resolved by SDS-PAGE and identified by mass spectrometry. The major 50 kDa band, observed in both sample...
ORGANISM(S): Xanthomonas citri pv. fuscans 
2023-05-10 | PXD037760 | Pride
Flic Xanthomonas citri (strain 306) and xanthomonas aurantifolii pathotype c (strain icmp 8435) Flagellin c 
ProteomicsEvolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria
Many bacteria decorate flagellin with sialic acid-like sugars such as pseudaminic acid (Pse) by O-glycosylation on serine or threonine residues. Evidence for sufficiency of sialylation by a conserved flagellin glycosyltransferase (fGT) system is lacking, presumably because of (a) missing component(s...
ORGANISM(S): Shewanella oneidensis (strain MR-1) Caulobacter crescentus (strain NA1000 / CB15N) 
2024-06-18 | PXD051215 | Pride
Pseudaminic acid Bacteria Glfm O-glycosylation Flagellin Maf 
ProteomicsRe-annotated role of beta-Galactosidase in Flagellin deglycosylation
Most Eukaryotes recognise flagellin as a signature of bacterial invasion. In contrast to animals, plants do not recognise flagellin proteins, but conserved peptides released from flagellin (Felix et al., 1999). However, these peptides (e.g. flg22) are folded and buried deeply inside the flagellin po...
ORGANISM(S): Nicotiana benthamiana Pseudomonas syringae pv. tomato 
2019-04-15 | PXD010461 | Pride
Viosamine Beta-galactosidase O-glycosylation Pseudomonas syringae Flagellin 
ProteomicsFlagellin (FliC) phosphorylation affects Type 2 protease secretion and biofilm dispersal in Pseudomonas aeruginosa PAO1
Protein phosphorylation has a major role in controlling the life-cycle and infection stages of bacteria. Proteome-wide occurrence of S/T/Y phosphorylation has been reported for many prokaryotic systems. Previously, we reported the phosphoproteome of Pseudomonas aeruginosa and Pseudomonas putida. In ...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2022-03-02 | PXD003645 | Pride
Phosphorylation Flagellin Lc-msms 
ProteomicsReleased peptide from flagellin cleavage by SBT5.2a
The aim of this experiment is to identify the released peptides from flagellin obtained afte cleavage and processing by SBT5.2
ORGANISM(S): Nicotiana benthamiana Pseudomonas amygdali pv. tabaci str. ATCC 11528 
2024-11-02 | PXD056369 | Pride
Plant immunity Pseudomonas syringae Subtilases Flg22 Flagellin Nicotiana benthamiana 
ProteomicsElucidation of the glycan structure of the b-type flagellin of Pseudomonas aeruginosa PAO1
Flagella are essential for motility and pathogenicity in many bacteria. The main component of the flagellar filament, flagellin, often undergoes post-translational modifications, with glycosylation being a common occurrence. In Pseudomonas aeruginosa PAO1, the b-type flagellin is O-glycosylated with...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2025-05-07 | PXD056085 | Pride
Glycosylation Mass spectrometry (ms) Proteomics Cell motility Bacteria Pseudomonas 
TranscriptomicsGene analysis of flagellin-treated nasal mucosa of Duox2-/- mice
To evaluate the roles of DUOX2 in flagellin- induced inflammatory response in mouse nasal mucosa. Wild type (Duox2+/+) and Duox2 knockout (Duox2-/-) mice were stimulated with 1 M-NM- 2 fold) were up-regulated in nasal mucosa of Duox2+/+ that was treated with fl...
ORGANISM(S): Mus musculus 
2013-12-18 | E-GEOD-26135 | biostudies-arrayexpress
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