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GenomicsTumor-specific retargeting of an oncogenic transcription factor chimera results in dysregulation of chromatin and transcription
Using EWS-FLI and its parental transcription factor, FLI1, we created a unique experimental system to address questions regarding the genomic mechanisms by which chimeric transcription factors cause cancer. We found that in tumor cells, EWS-FLI targets regions of the genome distinct from FLI1, despi...
ORGANISM(S): Homo sapiens 
2011-11-09 | E-GEOD-31838 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human Ewings sarcoma cell line A673 inducible-rescue experiment
A673 Ewing's sarcoma cells, with inducible EWS/FLI cDNA, harboring the EF-2-RNAi retrovirus, induced (or uninduced) for the indicated time period. Experiment Overall Design: Two replicates of a time course series, including the indicated time points, including both induced and uninduced samples.
ORGANISM(S): Homo sapiens 
2008-06-13 | E-GEOD-4563 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human Ewing sarcoma (A673) cells treated with cytarabine, doxorubicin and puromycin oral tongue squamous cell carcinomas to identify novel potential prognosticators
An increasing number of cancer-associated mutations have been identified. Unfortunately, little therapy today exploits these tumor-specific genetic lesions. Often, the resulting oncoproteins have been intractable to easy manipulation with current small molecule screening approaches. To overcome this...
ORGANISM(S): Homo sapiens 
2008-11-22 | E-GEOD-6930 | biostudies-arrayexpress
TranscriptomicsRNAi profiling of human Ewings sarcoma A673 Stable-Knockdown
A673 Ewing's sarcoma cells containing either control RNAi retroviral constructs (luc-RNAi), or RNAi retroviral constructs targeting the endogenous EWS/FLI fusion transcript (either EF-2-RNAi or EF-4-RNAi). Experiment Overall Design: Eight samples total. Four luc-RNAi, and four EWS/FLI knockdown con...
ORGANISM(S): Homo sapiens 
2008-06-13 | E-GEOD-4560 | biostudies-arrayexpress
ProteomicsHuman A673 Ewing sarcoma secretome - effect of EWS-FLI-1 silencing
EWS-FLI-1 was silenced by an shRNA in A673 Ewing sarcoma cells and the resulting alterations in the secretome was analyzed by GeLC-MS/MS approach (six gel slices for each sample, luciferase shRNA-expressing cell secretome as control)
ORGANISM(S): Homo sapiens (Human) 
2021-07-06 | PXD016052 | Pride
Ewing sarcoma Ews-fli-1 Gelc-ms/ms Secretome 
TranscriptomicsTranscription profiling of human Ewing sarcoma samples and EWS-FLI-1 inhibited Ewing sarcoma cell lines
The cellular origin of Ewing tumor (ET), a tumor of bone or soft tissues characterized by specific fusions between EWS and ETS genes, is highly debated. Through gene expression analysis comparing ETs with a variety of normal tissues, we show that the profiles of different EWS-FLI1-silenced Ewing cel...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7007 | biostudies-arrayexpress
ProteomicsImpact of media supplementation on a transcriptional activity of porcine embryos produced in vitro
The production of embryos in vitro caused a revolution in the field of assisted reproduction technologies, which currently allows the manipulation and selection of embryos before implantation. One of the key aspects of this process is the maturation of oocytes in a controlled in vitro environment. I...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2023-09-25 | PXD045481 | Pride
Dmem Maturation medium Oocyte Fli Porcine 
TranscriptomicsRUNX3 Facilitates Growth of Ewing Sarcoma Cells
Ewing sarcoma is an aggressive pediatric small round cell tumor that predominantly occurs in bone. Approximately 85% of Ewing sarcomas harbor the EWS/FLI fusion protein, which arises from a chromosomal translocation, t(11:22)(q24:q12). EWS/FLI interacts with numerous lineage-essential transcription...
ORGANISM(S): Homo sapiens 
2014-05-01 | E-GEOD-56900 | biostudies-arrayexpress
GenomicsThe FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [ATAC-seq]
The FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [ATAC-seq]
PRJNA674797 | ENA
GenomicsThe FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [CUT&RUN]
The FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [CUT&RUN]
PRJNA674798 | ENA
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