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GenomicsBlue‐shifting green fluorescent proteins using REPLACE
Blue‐shifting green fluorescent proteins using REPLACE
PRJNA1133788 | ENA
GenomicsFluorescent Mpox viruses
Generation of recombinant mpox viruses expressing fluorescent proteins
PRJEB82673 | ENA
ProteomicsScreening for novel immungenic proteins of Salmonella Enteritidis 125109
The screening of a cDNA derived expression library of Salmonella Enteritidis 125109 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (fimA) and negative (argC, gapA) reference p...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Enteritidis 
2013-10-01 | E-GEOD-49605 | biostudies-arrayexpress
OtherBlue‐shifting green fluorescent proteins using REPLACE
In the directed evolution experiments of EGFP or StayGold, the objective was to blue-shift the excitation light spectrum of GFP towards shorter wavelengths, resulting in an enhancement of fluorescence intensity upon 405 nm laser excitation. This facilitates the advancement of novel fluorescent prote...
ORGANISM(S): Mesocricetus auratus 
2024-09-30 | GSE271876 | GEO
ProteomicsScreening for novel immungenic proteins of Klebsiella pneumoniae MGH 78578
The screening of a cDNA derived expression library of Klebsiella pneumoniae MGH 78578 expressed in E.coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (ompA, mdh) and negative (pyrC, gapA) refer...
ORGANISM(S): Klebsiella pneumoniae 
2014-02-02 | E-GEOD-52536 | biostudies-arrayexpress
ProteomicsScreening for novel immunogenic proteins of Campylobacter jejuni NCTC 11168
The screening of a cDNA derived expression library of Campylobacter jejuni NCTC 11168 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. 1536 different clones were screened including positive (hisJ, cjaA, peb1a) and negative (argC, pyrC, ga...
ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 
2013-04-06 | E-GEOD-45268 | biostudies-arrayexpress
ProteomicsDetection of immunogenic proteins from bacteria
Detection of immunogenic proteins remains an important task for life sciences as it nourishes the understanding of pathogenicity, illuminates new potential vaccine candidates and broadens the spectrum of biomarkers applicable in diagnostic tools. Traditionally, immunoscreenings of expression librari...
ORGANISM(S): Campylobacter jejuni 
2011-10-26 | E-GEOD-33295 | biostudies-arrayexpress
ProteomicsmCherry contains a fluorescent protein isoform that interferes with its reporter function
Fluorescent proteins are essential reporters in cell biology and molecular biology. Here, we reveal that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform. The short isoform creates significant background fluorescence that bi...
ORGANISM(S): Escherichia coli 
2022-04-19 | PXD032954 | Pride
Lc-msms Mcherry 
TranscriptomicsQuantitative time-course profiling of Plasmodium falciparum transcripts and proteins throughout the 48-hour intraerythrocytic developmental cycle
Quantitative studies of the P. falciparum transcriptome have shown that the tightly controlled progression of the parasite through the intraerythrocytic developmental cycle (IDC) is accompanied by a continuous gene expression cascade where most expressed genes exhibit a single transcriptional peak. ...
ORGANISM(S): Plasmodium falciparum 
2011-06-07 | E-GEOD-24416 | biostudies-arrayexpress
GenomicsNear-infrared fluorescent reporter stem cell model
Near-infrared fluorescent reporter stem cell model
PRJEB51223 | ENA
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