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Cappable-seq was used to map transcription start sites globally in wild type Bacillus subtilis. Total RNA was isolated from cells grown in LB media until exponential phase. RNA corresponding to transcription start sites was capped with a 5' biotin tag, which was used for enrichment via a pull down w...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
Cappable-seq was used to map transcription start sites globally in B. subtilis and E. coli to investigate spurious transcription, and how B. subtilis prevents it.
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
Sequencing datasets produced during the investigation into the function of the CarD transcription factor in Rhodobacter sphaeroides. Sequencing datasets and in vitro biochemical assay reveal CarD is dependant on negative DNA supercoiling for activation of transcription. In this experiment, 4 sets ...
ORGANISM(S): Cereibacter sphaeroides 
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