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In our study, we employed activity-based protein profiling (ABPP), a technique that uses specialized inhibitors to identify active serine hydrolases in different strains of E. faecium (clade A1 and A2) and E. lactis under various growth conditions. Serine hydrolases, a large and diverse family of en...
ORGANISM(S): Enterococcus faecium Bacteria 
2024-06-22 | PXD048798 | Pride
Toxicity of dichlorvos (DDVP), an organophosphate (OP) pesticide, classically results from modification of the serine in the active sites of cholinesterases. However, DDVP also forms adducts on unrelated targets such as transferrin and albumin, suggesting that DDVP causes cellular perturbation by mo...
ORGANISM(S): Homo sapiens (Human) 
2014-07-31 | PXD001107 | Pride
Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae protease...
ORGANISM(S): Vibrio cholerae O1 str. VC91 
2016-02-22 | PXD003000 | Pride
Serine hydrolases play diverse roles in regulating host-pathogen interactions in a number of organisms, yet few have been characterized in the human pathogen Staphylococcus aureus. Here, we describe a chemical proteomic screen that identified 10 previously uncharacterized S. aureus serine hydrolases...
ORGANISM(S): Staphylococcus aureus 
2018-05-17 | PXD009210 | Pride
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