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UnknownDevelopment of a fritless packed column for capillary electrochromatography-mass spectrometry.
Not available
S-EPMC3249243 | biostudies-literature
ProteomicsStandard Pressure LC-MS/MS Using 1.9um C18 Particles Column at Room Temperature
Although the benefits of reduction of the size of reversed phase particles are established to provide increased sequencing depth and improved chromatography in LCMS experiments, the wide-scale adoption of optimally sized small particles in reversed-phase columns has been hampered by the necessity fo...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD002895 | Pride
Fritless column Jurkat Single-run proteomics Lc-ms/ms 
ProteomicsHighly reproducible improved label-free quantitative analysis of cellular phosphoproteome by optimization of LC-MS/MS gradient and analytical column construction
Expanding the sequencing depth of the peptides showing a statistically significant quantitative change arising from a biological stimulation is critical. Here we demonstrate that optimization of LC gradient and analytical column construction can reveal over 30,000 unique peptides and 23,000 phosphop...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD005650 | Pride
Human T cell Acute leukemia Q exactive Phosphoproteomics Label free 
UnknownHighly reproducible improved label-free quantitative analysis of cellular phosphoproteome by optimization of LC-MS/MS gradient and analytical column construction.
Not available
S-EPMC5542054 | biostudies-literature
ProteomicsCampylobacter concisus strain BAA-1547 GeLC-MSMS
Bacteria were washed three times in NaCl (150 mM). Cells were resuspended in 1 ml TSU buffer (50 mM Tris pH 8.0, 0.1% SDS, 2.5 M urea) and lysed by two freeze-thaw cycles in liquid nitrogen. Lysate proteins (40 ug) were separated and digested. Digested peptides were separated by nano-LC using an U...
ORGANISM(S): Campylobacter concisus (strain 13826) 
2014-04-28 | PXD000504 | Pride
Concisus Gelc-msms Campylobacter 
ProteomicsCampylobacter concisus UNSWCD GeLC-MSMS
Washed three times in NaCl (150 mM). Cells were resuspended in 1 ml TSU buffer (50 mM Tris pH 8.0, 0.1% SDS, 2.5 M urea) and lysed by two freeze-thaw cycles in liquid nitrogen. Lysate proteins (40 ug) were separated and digested. Digested peptides were separated by nano-LC using an Ultimate 3000 ...
ORGANISM(S): Campylobacter concisus UNSWCD 
2014-04-28 | PXD000505 | Pride
Concisus Gelc-msms Campylobacter 
ProteomicsProteomic analysis of Mycoplasma hyopneumoniae virulent strain 232
The experiment consists of M.hyp 232 cultures digests analyzed on two mass specs, LTQ Velos Pro and LTQ FT Ultra LTQ Velos Pro: Six cell culture replicates of M.hyo 232 were hydrophobically separated using tree detergents: Digitonin, Tween, and SDS. Each francion, including the insoluble pellet, wa...
ORGANISM(S): Mycoplasma hyopneumoniae (strain 232) 
2018-01-10 | PXD000118 | Pride
Mycoplasma hyopneumoniae 232 ltq velos ft mass spec proteomics 
ProteomicsCyanophora paradoxa muroplast LC-MSMS
Cyanophora paradoxa muroplasts were isolated on a Percoll gradient. Muroplasts were fractionated into soluble (stroma), envelope, and thylakoid proteins. Protein samples from envelope membranes, thylakoids, stroma and total muroplasts were separated by 12% SDS-PAGE. Each Protein lanewas cut into 10...
ORGANISM(S): Cyanophora paradoxa 
2012-10-30 | PXD000056 | Pride
Lc-msms Glaucophyte Cyanophora Plastid 
ProteomicsTemporary bioadhesion in the proseriate flatworm Minona ileanae
Flatworms can very rapidly attach to and detach from many substrates. In the presented work, we analyzed the adhesive system of the marine proseriate flatworm Minona ileanae. We used light-, scanning-, and transmission electron microscopy to analyze the morphology of the adhesive organs, which are l...
ORGANISM(S): Minona ileanae 
2019-11-12 | PXD013631 | Pride
Bioadhesion Flatworms Platyhelminthes 
ProteomicsLILRA3-GFP and GFP only Transfected HEK293T cells LC-MSMS
The leukocyte immunoglobulin-like receptor A3 (LILRA3) is a soluble protein primarily expressed by peripheral blood monocytes and is abundant in sera of healthy donors. Extracellular LILRA3 is anti-inflammatory and displays neuro-regenerative functions in vitro. However, its intracellular expression...
ORGANISM(S): Homo sapiens (Human) 
2021-04-08 | PXD024602 | Pride
Lc-msms Immunoprecipitation Hek293t cells Lilra3 
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