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ProteomicsSingle-cell proteomics of Arabidopsis leaf mesophyll protoplasts in the context of drought stress
We followed the Arabidopsis tape-sandwich protocol to isolate the leaf protoplast enriched with palisade mesophyll cells. Briefly, control and WD-stressed Arabidopsis rosette leaves were placed on 3M tape, and abaxial cell layers were peeled off by gently removing the tape, thereby exposing the adax...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-06-18 | MSV000095072 | MassIVE
ProteomicsMultimodal measurement of the transcriptome and proteome from dissociated human pancreatic islet cells using nanoSPLITS
Human pancreatic islets from two human donors (Prodo Laboratories) were acquired and cultured for 24 hr in pancreatic islet media (PIMS, Prodo Laboratories). Single cells were dissociated with TrypLE for ~15-30 minutes, washed with PBS, stained with Calcein-AM/propidium iodide, and sorted onto nanoS...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-07 | MSV000093330 | MassIVE
ProteomicsMultimodal measurement of the transcriptome and proteome in G2/M arrested single cells using nanoSPLITS
Single-cell proteomic data from RO-3306 treated alveolar type II epithelial cells (C10) and untreated C10 cells. Cells were sorted using cellenONE onto several nanoSPLITS chips. Cell lysates were then split for parallel RNAseq and proteomic analysis. For proteomics recovery experiment where both chi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-12-02 | MSV000090828 | MassIVE
ProteomicsTop-down proteomic analysis with FAIMS of Alzheimer's disease derived brain tissue
Proteomic datasets collected from a single human midfrontal cortex tissue sample. Tissue was homogenized in 8 M Urea, 10 mM ABC, and 10 mM TCEP before subsequent cleanup with 100K and 3K MWCO filters. NanoLC was performed for RP separation. Three technical replicates were collected without FAIMS. Se...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-14 | MSV000086696 | MassIVE
ProteomicsLarge scale, quantitative top-down proteomic analysis of human frontal gyrus brain tissue samples in the context of Alzheimer's disease
15-35 mg brain tissue plugs derived from dorsal lateral prefrontal cortex (DLPFC) were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homog...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-26 | MSV000093728 | MassIVE
ProteomicsDifferential solubilization and top-down proteomics with FAIMS control experiments applied to mouse brain
5-35 mg brain tissue plugs from mouse left or right hemisphere were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homogenization buffer (H...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-12-25 | MSV000093727 | MassIVE
ProteomicsEvaluating linear ion trap incorporation for MS3-based multiplexed single-cell proteomics
The project aims to evaluate the use of a linear ion trap to increase the sensitivity of multiplexed single-cell proteomics. Using diluted mouse peptide samples from C10 and Raw, we compared the proteome coverages, data missingness, and quantification performance between linear ion trap and Orbitrap...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-08-22 | MSV000090178 | MassIVE
ProteomicsCharacterization of cytokine treatment on human pancreatic islets by top-down proteomics
Top-down proteomic data from 6 human islet donors; islets were treated with interferon gamma (IFN-gamma) and interleukin-1 beta (IL-1 beta). Time points include t=0 (control) and t=24 hours for each donor. Samples were reduced and alkylated, then analyzed by LC-MS/MS. Data was searched with TopPIC u...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-05-06 | MSV000097810 | MassIVE
ProteomicsSpatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform
We demonstrate spatially resolved top-down proteomics (TDP) for proteoform identification and quantification from small sections of rat brain. We used the nanoPOTS (nanodroplet Processing in One pot for Trace Samples) platform, further improving the sample preparation by adding benzonase in the extr...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) Homo Sapiens (ncbitaxon:9606) 
2022-03-30 | MSV000089163 | MassIVE
UnknownEffect of alteplase on the CT hyperdense artery sign and outcome after ischemic stroke.
Not available
S-EPMC4731690 | biostudies-literature
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