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Transcriptomicssingle cell RNA sequencing of 120h mouse gastruloids cultured with different doses of retinol
We quantified the effects of retinol on the cell type composition of gastruloids at 120 hours after aggregation by single cell RNA sequencing (scRNA-seq). Gastruloids were generated from the Rarb2.LacZ reporter cell line and cultured according to the standard protocol and few modifications: - cultu...
ORGANISM(S): Mus musculus 
2026-07-24 | E-MTAB-17398 | biostudies-arrayexpress
TranscriptomicsBulk RNAseq of mouse gastruloids developed with homemade N2B27 and commercial NDiff227
Procedure: three independent experiments were done to generate three 96-well plates of E14-Tg2A gastruloids made with either homemade or commercial N2B27. 48 gastruloids per sample were used for RNA extraction using the Qiagen RNeasy Micro Kit. Library preparation and sequencing were done by the CRG...
ORGANISM(S): Mus musculus 
2025-04-01 | E-MTAB-14893 | biostudies-arrayexpress
TranscriptomicsSingle Cell RNA-seq time course of mouse gastruloids at 0h (mESCs), 24h, 48h and 72h post-aggregation
Single cell transcriptomic study (using 10x Genomics v3 kits) of gastruloids, aggregates of mESCs, at different developmental timepoints (24h, 48h and 72h post-aggregation). mESCs, corresponding to the 0h timepoint, were also sequenced. The aim of this study was to delineate the cell state transitio...
ORGANISM(S): Mus musculus 
2023-12-04 | E-MTAB-12749 | biostudies-arrayexpress
ProteomicsDeciphering lineage specification during early embryogenesis using multi-layered proteomics
Gastrulation is a critical stage of embryonic development during which the three germ layers are established. Deciphering the molecular mechanisms underlying this proces from a protein perspective remains a significant challenge. To address this, we employed a multilayered mass spectrometry-based pr...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-21 | PXD041309 | Pride
Proteomics Dtag system Interaction proteomics Enhancers Zeb2 Transcription factors Somitoids Gastruloids Developmental biology 
GenomicsCapturing cardiogenesis in gastruloids
Capturing cardiogenesis in gastruloids
PRJNA667329 | ENA
GenomicsSingle-cell and spatial transcriptomics reveal somitogenesis in gastruloids
Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids
PRJNA507788 | ENA
GenomicsRetinoic acid induces human gastruloids with posterior embryo-like structures
Retinoic acid induces human gastruloids with posterior embryo-like structures
PRJNA859692 | ENA
UnknownRetinoic acid induces human gastruloids with posterior embryo-like structures.
Not available
S-EPMC11469962 | biostudies-literature
ProteomicsHypoxia induces a transcriptional primitive streak signature in ES cells and enhances lineage patterning in gastruloids
The cellular microenvironment shapes stem cell identity and differentiation capacity. Mammalian early embryos are exposed to hypoxia in vivo and benefit from hypoxic culture in vitro. Yet, how different components of the hypoxia response impact stem cell transcriptional networks and lineage choices ...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-05 | PXD026641 | Pride
Embryonic stem Hypoxia Early embryos 
TranscriptomicsComparative single cell RNA seq of gastruloids from mESCs grown in 3 distinct media conditions facilitating distinct pluripotent states at 3 different developmental time points
Comparative single cell transcriptomic study (using 10x Genomics v3.1 kits) of gastruloids, aggregates of mESCs, at different developmental timepoints. mESCs, corresponding to the 0h timepoint, were also sequenced. The source mESCs were grown in 2 distinct media conditions: DMEM-based Serum-LIF (SL)...
ORGANISM(S): Mus musculus 
2024-11-13 | E-MTAB-13643 | biostudies-arrayexpress
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