Sort   by:  
 Page size 
ProteomicsLC-MS/MS-Based Proteomic Analysis of a Single Human-Derived Gel Band
LC-MS/MS-Based Proteomic Analysis of a Single Human-Derived Gel Band
ORGANISM(S): Homo sapiens (Human) 
2026-06-11 | PXD074137 | Pride
Xb10683gi 
TranscriptomicsUnraveling determinants of transcription factor binding outside the core binding site
Binding of transcription factors (TFs) to regulatory sequences is a pivotal step in the control of gene expression. Despite many advances in the characterization of sequence motifs recognized by TFs, our ability to quantitatively predict TF binding to different regulatory sequences is still limited....
ORGANISM(S): synthetic construct 
2015-03-04 | E-GEOD-66143 | biostudies-arrayexpress
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
TranscriptomicsBand-array detection of chromosomal rearrangements in diploid yeast cells exposed to ionizing radiation
At the organismal level, genome rearrangements are usually deleterious and are often associated with disease. Yet, on an evolutionary scale, they can be beneficial as they provide for rapid genetic diversification. DNA lesions, particularly double-strand breaks (DSBs), are sources of genome instab...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-6991 | biostudies-arrayexpress
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsProtein gel band method for improving the identification of stem proteins
Identifying the intracellular and cell wall-ionically bound glycoside hydrolases (GHs) carbohydrate esterases (CEs) at the late growth stage of Arabidopsis stems is important for understanding the mechanisms regulating the cell wall integrity. The fact that whole plant stems are used as biomass fee...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-05-11 | PXD001852 | Pride
Plant late growth stage Pectin-modifying enzyme Feedstock engineering Plant senescence Biomass recalcitrance Glycoside hydrolase Xylan-modifying enzyme Cell wall proteins 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
ProteomicsIdentification of a Zinc Carboxypeptidase in a crude lysate SDS-PAGE gel of a Porphyromonas gingivalis transcription regulator mutant
Porphyromonas gingivalis is one of the main etiological agents responsible for the initiation and progression of periodontal diseases. This bacterium inhabits unfavorable conditions, therefore, to adapt to the changing external environment, e.g. redox conditions, and nutrient limitations, it must re...
ORGANISM(S): Porphyromonas gingivalis ATCC 33277 Bacteria 
2025-03-27 | PXD057991 | Pride
Mse Lc-ms Sds-page Crp/fnr protein superfamily Gel band analysis Transcription regulator mutant Putative zinc carboxypeptidase Bottom-up Porphyromonas gingivalis 
ProteomicsCross-linked ammonia monooxygenase complex in BN-PAGE gel from Nitrososphaera viennensis
A band containing the ammonia monooxygenase complex from Nitrosospheara viennensis was cut from a Blue Native PAGE gel and cross linked using DSSO.
ORGANISM(S): Nitrososphaera viennensis EN76 
2023-02-13 | PXD034475 | Pride
Ammonia oxidizing archaea Ammonia monooxygenase Nitrification Copper membrane bound monooxygenase 
Sort   by:  
 Page size