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TranscriptomicsSystems Biology Analysis of Tenofovir 1% Gel in a Phase I Rectal Microbicide Trial
In MTN-007, a phase 1, randomized, double-blinded rectal microbicide trial, we used systems genomics/proteomics to determine the effect of tenofovir 1% gel, nonoxynol-9 2% gel, placebo gel or no treatment on rectal biopsies taken at baseline, after one application or after seven daily applications (...
ORGANISM(S): Homo sapiens 
2015-02-23 | E-GEOD-57025 | biostudies-arrayexpress
ProteomicsProtein Metabolism Underlying Heat Tolerance in Contrasting Creeping Bentgass Lines: Insights From Gel-Free Proteomics
One of the major disfunctions that occurs in heat-stressed plants is enhanced protein damage and a consequent decline in cellular protein content. Creeping bentgrass (Agrositis stolonifera L.) is an economically important perennial grass species which is largely used on high value turf areas but exp...
ORGANISM(S): Agrostis stolonifera 
2025-10-20 | PXD060809 | Pride
Heat stress  creeping bentgrass  polyubiquitin-omics  gel-free proteomics  proteostasis 
ProteomicsGel-free/label-free proteomic, biochemical and photosynthetic anaysis of cowpea resistance against Cowpea severe mosaic virus
In this present work, photosynthetic and biochemical parameters in conjunction with gel-free/label-free proteomic analysis were assessed in a resistant cowpea genotype challenged with CPSMV and compared with the corresponding data of the mock-inoculated control as an attempt to find out some clues t...
ORGANISM(S): Vigna unguiculata 
2024-09-12 | PXD005906 | Pride
Cowpea Incompatible interaction Physiology Biochemistry Gel-free/label-free proteomic 
ProteomicsTube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-18 | PXD003841 | Pride
Label-free quantitative proteomics Liquid digestion Sample preparation Tube-gel Stacking gel 
UnknownA Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection.
Not available
S-EPMC10000902 | biostudies-literature
TranscriptomicsEx vivo effects of Tenofovir on four vaginal epithelial cell lines
In MTN-007, a phase 1, randomized, double-blinded rectal microbicide trial, we used systems genomics/proteomics to determine the effect of tenofovir 1% gel, nonoxynol-9 2% gel, placebo gel or no treatment on rectal biopsies taken at baseline, after one application or after seven daily applications (...
ORGANISM(S): Homo sapiens 
2015-02-23 | E-GEOD-57026 | biostudies-arrayexpress
ProteomicsGel based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire
Plasma membrane (PM) encompasses total cellular contents, serves as a living barrier regulates all cellular exchanges in a spatiotemporal fashion. Most of the essential tasks of PMs including molecular transport, cell-cell interaction and signal transduction are carried out by their proteinaceous co...
ORGANISM(S): Cicer arietinum (Chickpea) (Garbanzo) 
2024-12-05 | PXD003697 | Pride
Shotgun proteomics Plant proteomics High resolution mass spectrometry 
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
UnknownQuantifying raft proteins in neonatal mouse brain by 'tube-gel' protein digestion label-free shotgun proteomics.
Not available
S-EPMC2045652 | biostudies-literature
ProteomicsGel-Free/Label-Free Proteomic Analysis Uncovers the Roles of Endoplasmic Reticulum of Soybean Root Tip under Flooding and Drought Stresses
As a stresses sensitive crop, this study will give some clues to enhance soybean tolerance of flooding and drought stresses. To uncover the response mechanisms occurring in the ER triggered by flooding and drought, ER proteomics was performed using gel-free/label-free proteomic approach. Besides, gl...
ORGANISM(S): Glycine max 
2017-04-03 | PXD003397 | Pride
Proteomics Drought Endoplasmic reticulum Root tip Soybean Flooding 
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