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Cells were grown aerobically at 37 degC in Spizizen minimal medium either supplemented with 1 mM glycine betaine, 1.2 M NaCl or 1.2 M NaCl and 1 mM glycine betaine. Cells were harvested in the exponential growth phase at an OD600nm of 1.0. Proteome analysis was performed with samples from three biol...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-01-08 | MSV000084766 | MassIVE
We used cDNA generated from total mRNA for RNA-Seq analysis (Genome Analyzer II at GATC Biotech AG) to monitore the gene expression of P. putida after a cold shock from 30M-BM-0C to 10M-BM-0C Culture was grown in minimal medium supplemented with succinate at 30M-BM-0C. In mid-exp. phase cells were ...
ORGANISM(S): Pseudomonas putida KT2440 
We present a comparative analysis of sex-specific tegument proteins of paired or virgin Schistosoma mansoni. By apply a new and highly sensitive workflow, detection of even low abundance proteins from the worm was achieved Therefore, a streptavidin-biotin affinity purification technique in combinati...
ORGANISM(S): Schistosoma mansoni (Blood fluke) 
2022-05-20 | PXD031167 | Pride
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