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ProteomicsFast Profiling of Protease Specificity reveals similar substrate specificities for cathepsins K, L and S
N-terminal COFRADIC analysis of cathespine K, L and S to obtain the substrate specificity profile of these cysteine cathepsins.
ORGANISM(S): Homo sapiens (Human) 
2015-02-10 | PXD001536 | Pride
Cathespins N-terminal cofradic 
ProteomicsMetabolic regulation of N-terminal acetylation in yeast
N-terminal (Nt) acetylation, catalyzed by N-terminal acetyltransferases (NATs), has emerged as an important co-translational modification in eukaryotes, and involves the transfer of the acetyl moiety from acetyl-CoA (Ac-CoA) to the α-amino group of a nascent polypeptide. Here, we report the first gl...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-09-07 | PXD004326 | Pride
Metabolism Acetyl coenzyme a Saccharomyces cerevisiae N-terminal acetylation N-terminomics 
UnknownCombined Mapping of Multiple clUsteriNg ALgorithms (COMMUNAL): A Robust Method for Selection of Cluster Number, K.
Not available
S-EPMC4652212 | biostudies-literature
ProteomicsSubstrate specificity divergence of granzyme tryptases.
Elucidating the true physiological functions of granzymes has been challenging since the origin of the granzyme studied, the use of non-physiological granzyme concentrations and the interspecies use of granzymes, with extrapolation of all these data to orthologous granzymes has led to inconsistencie...
ORGANISM(S): Homo sapiens (Human) 
2015-09-30 | PXD000570 | Pride
Proteomics Granzyme N-terminal cofradic Protease Specificity profile 
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
ProteomicsGrM - Human and mouse granzyme M display divergent and species-specific substrate specificities
Complementary positional proteomics of human and mouse granzyme M treated K-562 lysates
2012-05-23 | PRD000347 | Pride
ProteomicsProteomic profiling of cathepsin specificity by FPPS
Identification of protease substrates and determination of their specificities can provide important insights into their function. In the past decade, several proteomic approaches for identification of protease cleavage sites were developed, each relying on various strategies for peptide labelling a...
ORGANISM(S): Homo sapiens (Human) 
2015-02-10 | PXD001553 | Pride
Degradomics Proteases Cathepsins 
ProteomicsProteomic data and structure analysis combined reveal interplay of structural rigidity and flexibility on selectivity of cysteine cathepsins
Addressing the elusive specificity of cysteine cathepsins, which in contrast to caspases and trypsin-like proteases lack strict specificity determining P1 pocket, was calling for innovative approaches. Proteomic analysis of cell lysates with human cathepsins K, V, B, L, S, and F identified 30,000 cl...
ORGANISM(S): Homo sapiens (Human) 
2023-03-29 | PXD041185 | Pride
Imixpro Cofradic N-terminomics Cathepsins 
ProteomicsA COFRADIC protocol to study protein ubiquitination
We here apply the COmbined FRActional DIagonal Chromatography (COFRADIC) technology to enrich for ubiquitinated peptides and identify sites of ubiquitination by mass spectrometry. Our technology bypasses the need to ectopically overexpress tagged variants of ubiquitin and the use of sequence-biased ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080780 | MassIVE
UnknownIdentification and characterization of immunogenic proteins of mycoplasma genitalium.
Not available
S-EPMC1539121 | biostudies-literature
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