Sort   by:  
 Page size 
ProteomicsComprehensive cross-linking mass spectrometry reveals molecular insights into the between peripheral and core NuRD complex subunits
Affinity purifications of CHD4 and CDK2AP2 to identify their interactors. The C-terminally tagged CHD4 has a C-terminal truncation to test whether this domain is required for the interaction with other NuRD subunits. GFP-purifications of both N- and C-terminally tagged CDK2AP2 were performed.
ORGANISM(S): Homo sapiens (Human) 
2020-12-07 | PXD017378 | Pride
Chd Cdk2ap2 Doc1r Nurd 
ProteomicsFungal COP9 signalosome assembly requires connection of two trimeric intermediates for integration of intrinsic deneddylase
The eight-subunit COP9 signalosome (CSN complex), controls the exchange of E3 ubiquitin cullin RING ligase receptors through its deneddylase activity, providing specificity to eukaryotic protein degradation. The conserved eight-subunit CSN complex is required for multicellular development of the fil...
ORGANISM(S): Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) 
2023-08-10 | PXD043205 | Pride
Cop9 signalosome Aspergillus nidulans Gfp-affinity purifications Csn complex Native complex assembly Subcomplexes 
ProteomicsThe interactome of a family of potential methyltransferases
Human methytransferase like proteins (METTL) are part of a large protein family characterized by the presence of binding domains for S-adenosyl methionine, a co-substrate in most methylation reactions. Despite the fact that members of this protein family were shown or predicted to be DNA, RNA or pro...
ORGANISM(S): Homo sapiens (Human) 
2019-05-06 | PXD011125 | Pride
Gfp affinity purifications Interaction proteomics Mass spectrometry Mettl 
ProteomicsATG8 interactions in pexophagy conditions
In the project «ATG8 interactions in pexophagy conditions» anti-GFP affinity purifications (APs) of cells expressing (a) GFP as negative control, (b) GFP-GABARAP, (c) GFP-MAP1LC3B (LC3) were performed (each n=3 biol. replicates).
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD048003 | Pride
Autophagy Gabarap Affinity purification Map1lc3b Lc-ms/ms 
UnknownImproved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.
Not available
S-EPMC3605737 | biostudies-literature
ProteomicsEvaluation of the effects of sample size on affinity purification-mass spectrometry
An affinity purification experiment was carried out to evaluate the effects of sample size on the performance of AP-MS experiments. The Arp2/3 complex was affinity purified from Saccharomyces cerevisiae cells containing GFP-tagged Arc35, a core subunit of the complex, in-gel digested by trypsin, and...
ORGANISM(S): Saccharomyces Cerevisiae By4741 (ncbitaxon:1247190) 
2020-06-08 | MSV000085564 | MassIVE
UnknownIdentification of Drosophila centromere associated proteins by quantitative affinity purification-mass spectrometry.
Not available
S-EPMC4536286 | biostudies-literature
TranscriptomicsTDP-43 Interactor and Motor Neuron Tagged Ribosome Affinity Purification in a Drosophila Model of ALS
We report the enrichment of mRNAs with motor neuron specific TDP-43 and tagged ribosomes in control as well as multiple models of TDP-43 induced neurodegeneration. Amyotrophic lateral sclerosis (ALS) is a genetically heterogeneous neurodegenerative disease in which 97% of patients exhibit cytoplasmi...
ORGANISM(S): Drosophila melanogaster 
2021-03-31 | GSE156222 | GEO
ProteomicsProteins associated wth Trypanosoma brucei HNRNPF/H
We aimed to find proteins associated with HNRNPH in bloodstream-form _Trypanosoma brucei_. We integrated a sequence encoding a cleavable TAP (Tandem Affinity Purification) tag into the genome upstream of, and in frame with, one _HNRNPH_ gene. The other allele was deleted. TAP-HNRNPH was purifed fro...
ORGANISM(S): Trypanosoma brucei 
2022-10-15 | PXD037289 | Pride
Trypanosoma brucei  mrna  rna-binding protein  trans splicing  mrna decay 
ProteomicsAP-SWATH analysis reveals a direct involvement of VCP/p97 in integrated stress response signalling through CReP/PPP1R15B degradation
The ubiquitin-directed AAA-ATPase VCP/p97 facilitates degradation of misfolded proteins in diverse cellular stress response pathways. Resolving the complexity of dynamic interactions with hosts of accessory proteins and substrates has therefore been a major challenge. Here, we used affinity-purifica...
ORGANISM(S): Homo sapiens (Human) 
2018-04-04 | PXD007876 | Pride
Integrated stress response Ap-swath P97 
Sort   by:  
 Page size