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ProteomicsAP-SWATH analysis reveals a direct involvement of VCP/p97 in integrated stress response signalling through CReP/PPP1R15B degradation
The ubiquitin-directed AAA-ATPase VCP/p97 facilitates degradation of misfolded proteins in diverse cellular stress response pathways. Resolving the complexity of dynamic interactions with hosts of accessory proteins and substrates has therefore been a major challenge. Here, we used affinity-purifica...
ORGANISM(S): Homo sapiens (Human) 
2018-04-04 | PXD007876 | Pride
Integrated stress response Ap-swath P97 
UnknownAP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation.
Not available
S-EPMC6030730 | biostudies-literature
ProteomicsTCTP interactome analysis by AP-SWATH
TCTP binding proteins were identified by immunoprecipitation using anti-FLAG antibody and SWATH acquisition.
ORGANISM(S): Homo Sapiens (human) 
2018-11-05 | PXD007578 | JPOST Repository
ProteomicsEF1A1 and EF1A2 interactome analysis by AP-SWATH
EF1A1- and EF1A2-binding proteins were identified by immuno-precipitation using anti-FLAG antibody and SWATH.
ORGANISM(S): Homo Sapiens (human) 
2018-11-05 | PXD007604 | JPOST Repository
ProteomicsSWATH analysis of 1433B_HUMAN affinity purifications from HEK293 cells after time course treatment with IGF1
Protein complexes and protein interaction networks are essential mediators of most biological functions. Complexes supporting transient functions such as signal transduction processes are frequently subject to dynamic remodeling. Currently, the majority of studies into the composition of protein com...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-20 | MSV000082833 | MassIVE
ProteomicsSWATH analysis of 1433B_HUMAN affinity purifications from HEK293 cells after time course treatment with IGF1
Protein complexes and protein interaction networks are essential mediators of most biological functions. Complexes supporting transient functions such as signal transduction processes are frequently subject to dynamic remodeling. Currently, the majority of studies into the composition of protein com...
ORGANISM(S): Homo sapiens (Human) 
2013-09-25 | PXD000343 | Pride
Ap-ms Ap-swath Quantitative interaction proteomics Affinity purificaiton 
ProteomicsDynamic of the Grb2 interactome in primary cells by DIA-MS
We applied AP-SWATH to quantify Grb2 interaction dynamics in primary T cells with a high level of reproducibility and accuracy. We concluded that AP-SWATH is a robust approach to quantify signaling dynamics in primary cells and could be further expanded to additional hub proteins using a range of ce...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-09 | PXD003972 | Pride
Swath Dia-ms Primary t cells Grb2 Interactome 
ProteomicsDynamic of the Grb2 interactome in primary cells by DDA-MS
We applied AP-MS to quantify Grb2 interaction dynamics in primary T cells. Data generated here in shotgun/DDA mode were used to build a high quality Grb2 binders-specific SWATH assay library for high-throughput targeted analysis of DIA data.
ORGANISM(S): Mus musculus (Mouse) 
2017-03-09 | PXD003973 | Pride
Primary t cells Ms Grb2 Interactome Dda 
ProteomicsMulti-layered proteomic analyses decode compositional and functional effects of cancer mutations on kinase complexes
With the rapidly increasing availability of genomic data and ensuing identification of disease associated mutations, the determination of molecular mechanisms by which such mutations affect biochemical processes and phenotypes remains a major challenge. In this study we developed and applied a multi...
ORGANISM(S): Homo sapiens (Human) 
2020-07-22 | PXD015687 | Pride
Human Prm Dyrk2 Swath-ms Dda Cancer mutations 
ProteomicsJPST000107 TCTP interactome analysis by AP-SWATH [Reanalysis: JPST000107]
TCTP binding proteins were identified by immunoprecipitation using anti-FLAG antibody and SWATH acquisition. [Original project description]
ORGANISM(S): Homo Sapiens (human) 
2023-04-01 | RPXD034324 | JPOST Repository
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