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ProteomicsArabidopsis Wdr8 interactors identification
To identify Arabidopsis Wdr8 interactors, immunoprecipitation associated LC-MS/MS analysis was carried out. Crude proteins extracted from Wdr8-GFP expressing transgenic plants was subjected to the immuno-precipitation assay using GFP antibody magnetic beads (µMACS GFP isolation kit, Miltenyi Biotec)...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-17 | PXD026063 | Pride
Gfp antibody coupled immunoprecipitation Arabidopsis seedlings Lc-ms/ms 
GenomicsIdentification of Transcription Factor EGL-5::GFP Binding Regions in L3
modENCODE_submission_3159 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2011-03-31 | E-GEOD-25796 | biostudies-arrayexpress
GenomicsIdentification of Transcription Factor PHA-4::GFP Binding Regions in YA
modENCODE_submission_3161 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2011-03-31 | E-GEOD-25799 | biostudies-arrayexpress
ProteomicsIdentification of FLS2 interacting proteins by co-immunoprecipitation and shot gun proteomics
To identify immediate early signaling components in the FLS2 pathway we performed co-immunoprecipitation (co-IP) experiments with FLS2-GFP as bait in Arabidopsis. We immunoprecipitated FLS2-GFP with anti-GFP antibody coated beads and analyzed co-precipitated proteins by liquid chromatography tandem ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-01-26 | PXD003189 | Pride
Co-immunoprecipitation Arabidopsis Fls2 Immunity Protein-protein interaction Pattern recognition receptor Signaling Plasma membrane 
ProteomicsIdentification of phosphorylation sites in human IGF2BP1 by immunoprecipitation coupled to mass spectrometry
Many RNA-binding proteins are regulated by posttranslational modifications, most commonly phosphorylation. IGF2BP1 is an RNA-binding protein, which regulates protein stability, translation and localization. Moreover, it was proposed to regulate mRNA stability and translation during proteotoxic stres...
ORGANISM(S): Homo sapiens (Human) 
2024-11-13 | PXD045761 | Pride
Q exactive hf-x Human Igf2bp1 Proteotoxic stress Hek293t Igf2bp3 
GenomicsIdentification of Transcription Factor MEP-1::GFP Binding Regions in Embryos
modENCODE_submission_2600 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2011-02-18 | E-GEOD-25800 | biostudies-arrayexpress
TranscriptomicsGenome-wide mapping of BES1 binding sites in Arabidopsis thaliana
Brassinosteroids (BRs) are important regulators for plant growth and development. BRs signal to control the activities of the BES1 and BZR1 family transcription factors. In order to further understand the mechanism by which BES1/BZR1 regulates downstream genes, we performed chromatin immunoprecipita...
ORGANISM(S): Arabidopsis thaliana 
2011-01-02 | E-GEOD-24684 | biostudies-arrayexpress
GenomicsPfAP2-I-GFP anti-GFP and anti-IgG ChIP-seq
To determine the genome-wide occupancy of the Plasmodium falciparum transcriptional regulator of invasion PfAP2-I (PfDd2_100013100/PF3D7_1007700), we used chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq). Synchronized, schizont stage, 40 hours post-invasion, cultures ...
ORGANISM(S): Plasmodium falciparum 
2017-06-01 | GSE80293 | GEO
GenomicsIdentification of Transcription Factor SKN-1::GFP Binding Regions in L1
modENCODE_submission_2622 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2011-02-18 | E-GEOD-25810 | biostudies-arrayexpress
ProteomicsSubclass I SnRK2-interacting protein, LC-MS/MS
To reveal subclass I SnRK2-interacting proteins, we conducted co-immunoprecipitation using anti-GFP antibody from subclass I SnRK2-GFP expressing plants. Immunoprecipitates were identified by LC-MS/MS analyses.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-04-27 | PXD017371 | Pride
Snrk2 Arabidopsis Interactome Lc-ms/ms 
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