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UnknownStructure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification.
Not available
S-EPMC7148334 | biostudies-literature
ProteomicsArabidopsis Circadian Clock RVE8 LNK1 LNK2 Interactome LC-MS
The timing of many molecular and physiological processes in plants occurs at a specific time of day. The circadian MYB-like transcription factor REVEILLE 8 (RVE8) interacts with its transcriptional coactivators NIGHT LIGHT INDUCIBLE AND CLOCK REGULATED 1 (LNK1) and LNK2 to promote the expression of ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-04-24 | PXD034089 | Pride
Arabidopsis thaliana Affinity purification Circadian clock Lcms 
ProteomicsArabidopsis endomembrane enrichments: Affinity enrichment of GFP bait
Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), E...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-04-22 | PXD001885 | Pride
Arabidopsis Clathin Affnity purification Small gtpases 
ProteomicsAffinity Purification of ISWI chromatin remodeler in Trypanosoma cruzi
Chromatin remodeling is a general mechanism involved in DNA-dependent processes, including gene expression regulation. ISWI ATPase is the catalytic subunit in diverse chromatin remodeling complexes that changing histone–DNA interactions and therefore play a pivotal role in different nuclear processe...
ORGANISM(S): Trypanosoma cruzi Dm28c 
2020-05-22 | PXD017869 | Pride
Affinity purification Iswi Trypanosoma cruzi Lc-ms/ms 
ProteomicsArabidopsis endomembrane enrichments: Affinity enrichment of CLC2-GFP in WS-2
Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), E...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-04-22 | PXD001880 | Pride
Arabidopsis Clathin Affnity purification Small gtpases 
ProteomicsVEX1-GFP Pull-downs - Affinity-purified VEX1GFP-associated proteins using cryomilling and high-affinity nanobodies
We affinity-purified VEX1GFP-associated proteins using cryomilling and high-affinity nanobodies. The procedure was carried out initially in insect-stage T. brucei, for which protocols were established, and then in bloodstream-form T. brucei. Quantitative proteomic analysis revealed tag-dependent enr...
ORGANISM(S): Trypanosoma brucei 
2019-05-31 | PXD013304 | Pride
Vex1  vsg  allelic exclusion  trypanosoma brucei 
UnknownVectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium.
Not available
S-EPMC1885977 | biostudies-literature
ProteomicsZNF432 stimulates PARylation and inhibits DNA resection to balance PARPi sensitivity and resistance
Affinity-purification coupled to mass spectrometry of GFP-ZNF432 expressed in wild-type and PARP1 KO 293T cells (with and without H2O2-induced DNA damage and PARP1 activation).
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD038731 | Pride
Parp1 Dna repair Parp inhibitors Homologous recombination Znf432 
ProteomicsTAZ (WWTR1) Interactome Analysis using a GBP nanotrap based affinity purification approach
The transcriptional co-activator TAZ (WWTR1) plays a crucial role in regulating gene expression related to cell proliferation, differentiation, and tissue homeostasis, particularly within the Hippo signaling pathway. Since TAZ does not directly bind to DNA, it largely relies on its protein-protein i...
ORGANISM(S): Mus musculus (Mouse) Rattus norvegicus (Rat) 
2025-02-11 | PXD060532 | Pride
Mass spectrometry Affinity-purification Skeletal muscle Taz Nanobody 
TranscriptomicsComparative translating ribosome affinity purification-RNAseq (TRAPseq) by lung cell-specific drivers
Translating ribosome affinity purification coupled to next-generation RNA sequencing permits profiling of specific cellular compartments. Here, we used well-characterized Cre and CreER drivers to lineage trace different cell populations in the developing lung with a ribosomal L10-GFP fusion protein....
ORGANISM(S): Mus musculus 
2025-12-01 | GSE250140 | GEO
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