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We affinity-purified VEX1GFP-associated proteins using cryomilling and high-affinity nanobodies. The procedure was carried out initially in insect-stage T. brucei, for which protocols were established, and then in bloodstream-form T. brucei. Quantitative proteomic analysis revealed tag-dependent enr...
ORGANISM(S): Trypanosoma brucei 
2019-05-31 | PXD013304 | Pride
ChIP-Seq analysis of Myc-tagged VEX1
RNA-seq following knockdown of VEX2, VEX1/VEX2 or CAF-1b
The VEX complex regulates mVSG expression during the transition to mammalian infectivity in Trypanosoma brucei
UPF1-like helicases play roles in telomeric heterochromatin formation and X-chromosome inactivation and we focus here on variant surface glycoprotein (VSG) exclusion-factor-2 (VEX2), a UPF1-related protein in the African trypanosome. This bloodstream parasite evades host immunity by employing monoge...
ORGANISM(S): Trypanosoma brucei 
2023-11-20 | PXD040686 | Pride
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