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ProteomicsDNMT3A regulates megakaryocyte-biased HSC fate decisions
We characterized the role of the DNA methyltransferase DNMT3A in the fate decisions of murine megakaryocyte-biased HSCs and in the differentiation of megakaryocytes and platelets. In this experiment, we isolated washed platelets from 4 WT and 4 Dnmt3a+/– germline mice (2 males and 2 females per grou...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-09 | PXD055265 | Pride
Platelets Megakaryocytes Dna methylation Dnmt3a 
GenomicsGlobal methylation alterations in Dnmt3aKO derived malignancies
To test the long-term effects of lack of Dnmt3a on mouse HSCs, we established a cohort of lethally irradiated mice transplanted with Dnmt3a-KO or WT HSC control cells. Dnmt3a deletion in purified HSCs transplanted alone leads to an array of hematologic disorders that models the spectrum of disorders...
ORGANISM(S): Mus musculus 
2014-12-08 | E-GEOD-60264 | biostudies-arrayexpress
GenomicsLarge conserved domains of low DNA methylation maintained by 5-hydroxymethycytosine and Dnmt3a (CMS bisulfite-seq)
Gains and losses in DNA methylation are prominent genomic features of all mammalian cell types. To gain insight into mechanisms that could promote shifts in DNA 5-hydroxymethylation (5hmc) patterns and thus contribute to cell fate, including malignant transformation, we performed genome-wide mappin...
ORGANISM(S): Mus musculus 
2014-01-22 | E-GEOD-53742 | biostudies-arrayexpress
TranscriptomicsExpression profiling of Irgm1-/- (Lrg-47) HSCs
To assess gene expression changes in Irgm1 (Lrg-47) deficient HSCs Keywords: genetic modification, knockout A total of 6 samples were analyzed: 3 experimental replicates of Irgm1 HSCs were compared with 3 control replicates of WT HSCs
ORGANISM(S): Mus musculus 
2011-06-22 | E-GEOD-11591 | biostudies-arrayexpress
ProteomicsPeptidylprolyl isomerase A (PPIA) protein complex
Identification of PPIA protein complex using wild type and mutant cell lines.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-05-27 | MSV000083867 | MassIVE
ProteomicsTMT isobaric profiling of young versus old haematopoietic stem cells
Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spec...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-10-16 | MSV000093126 | MassIVE
ProteomicsPulsed SILAC study of cell lines following PPIA knockdown
Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spec...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-16 | MSV000093125 | MassIVE
ProteomicsTMT isobaric profiling of PPIA heterozygous versus knockout haematopoietic stem cells
Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spec...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-10-16 | MSV000093127 | MassIVE
Genomicsde novo DNA Methylation Balances Hematopoietic Stem Cell Self-Renewal and Differentiation
Cytosine methylation is an epigenetic mark usually associated with gene repression. Despite a requirement for de novo DNA methylation for differentiation of embryonic stem cells, its role in somatic stem cells is unknown. Using conditional ablation, we show that loss of either, or both, Dnmt3a or Dn...
ORGANISM(S): Mus musculus 
2011-09-30 | E-GEOD-27322 | biostudies-arrayexpress
ProteomicsMouse Bone Marrow Proteome Profiling
Hematopoietic stem cells give rise to all blood lineages, can fully re-populate the bone marrow, and easily outlive the host organism. To better understand how stem cells remain fit during aging, we analyzed the proteome of hematopoietic stem and progenitor cells.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2019-05-23 | MSV000083845 | MassIVE
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