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TranscriptomicsRNA-seq of Saponaria officinalis tissues (leaves, stems and roots) in response to MOCK or 50 uM Methyl Jasmonate treatment at 6 and 24 hours
The experiment aims to identify genes encoding enzymes involved in the biosynthesis of triterpenoid saponins in Saponaria officinalis. RNA-seq data generated were generated from leaves, stems, and roots from 3 months old- hydroponically grown S. officinalis plants that were treated with 50 µM methyl...
ORGANISM(S): Saponaria officinalis 
2026-01-15 | E-MTAB-14773 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis of Arabidopsis thaliana cells treated with RN1 and 2,4-D
A PSB-D wild type Arabidopsis thaliana cell culture (Arabidopsis Biological Resource Center stock: CCL84840) was grown after subculturing for one week and divided into separate 10 mL samples for 24 h. Samples were then treated with 50 µM RubNeddin1 (RN1, Chembridge ID 6389186), 2,4-D, or an equal vo...
ORGANISM(S): Arabidopsis thaliana 
2021-08-03 | E-MTAB-7761 | biostudies-arrayexpress
ProteomicsDiscovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry
Jasmonic acid (JA) is the precursor of the plant hormone, jasmonoyl-isoleucine, with roles in defences and development. Molecular signalling pathways of JA are well-understood, but it is still not fully understood how JA is metabolised. We used limited proteolysis-coupled mass spectrometry to search...
ORGANISM(S): Solanum lycopersicum 
2026-08-27 | PXD057493 | Pride
Jasmonic acid Lip-ms Protein-metabolite interactome Tomato 
TranscriptomicsThe effect of loss of Makibishi1-(MKB1) function on the transcriptome of Medicago truncatula hairy roots
RNA-Seq analysis on Medicago truncatula hairy roots, either control lines or lines silenced for the Makbishi1 (MKB1) gene, grown in either mock conditions or elicited with the plant stress hormone methyl jasmonate for 4 hours
ORGANISM(S): Medicago truncatula 
2022-05-07 | E-MTAB-11668 | biostudies-arrayexpress
TranscriptomicsEarly nutrient starvation response of the diatom Phaeodactylum tricornutum
An experiment was performed to determine the similarities on the RNA level between different conditions where cell division stops in the diatom Phaeodactylum tricornutum. Many of these conditions also increase the accumulation of lipids within the cell or impair photosynthesis. The different metabol...
ORGANISM(S): Phaeodactylum tricornutum 
2015-12-07 | E-MTAB-4096 | biostudies-arrayexpress
TranscriptomicsRoot transcriptome of ninja-1
A major role of NINJA is to repress root jasmonate signalling and allow normal cell elongation. To further characterize the transcriptional changes occurring in the root in the absence of a NINJA-dependent repression mechanism, we conducted a microarray analysis of ninja-1 versus WT roots. Root tran...
ORGANISM(S): Arabidopsis thaliana 
2015-05-28 | E-GEOD-65840 | biostudies-arrayexpress
TranscriptomicsRhizogenic Agrobacterium protein RolB interacts with the TOPLESS and NINJA repressor proteins to reprogram plant jasmonate signaling.
RNA sequencing was performed to compare the gene expression in tomato roots upon infection with the wild-type rhizogenic Agrobacterium K599 strain, the rolB∆ mutant strain and the rolB∆ strain with the constructs expressing either the native rolB oncogene or the rolBmEAR2 mutant.
ORGANISM(S): Solanum lycopersicum 
2022-12-05 | E-MTAB-12405 | biostudies-arrayexpress
TranscriptomicsRNAseq analysis of wild type dark grown PSB-D cell cultures
RNA was isolated from wild-type dark grown PSB-D cell cultures in three biological repeats using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega). A Trueseq RNA-Seq library (Illumina) was compiled and sequenced as 50-bp single read using Illumina HiSeq 2000.
ORGANISM(S): Arabidopsis thaliana 
2017-08-07 | E-MTAB-3915 | biostudies-arrayexpress
TranscriptomicsRhizogenic Agrobacterium protein RolB interacts with the TOPLESS and NINJA repressor proteins to reprogram plant jasmonate signaling (rolB overexpression)
RNA sequencing was performed to compare the gene expression in tomato hairy root lines transformed with the inducible rolB, rolBmEAR2 or GFP overexpression constructs, 24 h after mock and β-estradiol treatments. This set-up allowed us to map the general RolB targets as well as the specific targets o...
ORGANISM(S): Solanum lycopersicum 
2022-07-08 | E-MTAB-11880 | biostudies-arrayexpress
TranscriptomicsTSAR1 and TSAR2 activate triterpene saponin production in Medicago truncatula
We identified two novel transcription factors ‘Triterpene Saponin Activation Regulator’ (TSAR) 1 and 2 that each activate a specific branch of saponin synthesis in M. truncatula. Therefore we generated three independent TSAR1 and three independent TSAR2 overexpression (OE) hairy root lines. As a con...
ORGANISM(S): Medicago truncatula 
2017-02-15 | E-MTAB-3532 | biostudies-arrayexpress
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