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ProteomicsIdentification of Glycosylphosphatidylinositol Anchored Proteins and ω-sites Using TiO2-Based Affinity Purification
Glycosylphosphatidylinositol anchored proteins (GPI-APs) in the outer leaflet of the membrane microdomains, commonly referred to as lipid rafts, play important roles in many biological processes. To enable large-scale site-specific analysis of GPI anchoring, we developed a method for identification ...
ORGANISM(S): Homo sapiens (Human) 
2016-05-27 | PXD003105 | Pride
Gpi anchor 
TranscriptomicsGPI-anchored Timp1 protein
Tissue inhibitor of metalloproteinase 1 (TIMP-1) controls matrix metalloproteinase (MMP) activity through 1:1 stochiometric binding. Human TIMP-1 fused to a glycosylphosphatidylinositol (GPI) anchor (TIMP-1-GPI) shifts the activity of TIMP-1 from the extracellular matrix to the cell surface. TIMP-1-...
ORGANISM(S): Homo sapiens 
2012-08-23 | E-GEOD-40301 | biostudies-arrayexpress
ProteomicsExtensive proteomic approaches to identify glycosylphosphatidylinositol-anchored proteins in Arabidopsis reveal their diverse responses to cold acclimation
When plants are exposed to non-freezing low temperatures, they can increase their freezing tolerance (cold acclimation, CA). Changes of plasma membrane (PM) and sterol/sphingolipid-enriched microdomain proteins are thought to be crucial for acquisition of freezing tolerance. Particularly, glycosylph...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-07-29 | PXD002908 | Pride
Arabidopsis Plasma membrane Detergent-resistant membrane Microdomain Gpi-anchored protein Apoplast Freezing tolerance Cold acclimation 
ProteomicsA novel structure of GPI anchored proteins-NT5E
GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is atta...
ORGANISM(S): Homo sapiens (Human) 
2022-05-31 | PXD029850 | Pride
Nt5e Cd59 Pigo Pigg Pigb 
ProteomicsA novel structure of GPI anchored proteins NTNG2
GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is atta...
ORGANISM(S): Homo sapiens (Human) 
2022-05-31 | PXD029851 | Pride
Pigg pigb pigo ntng2 
GenomicsThe Histone Methyltransferase Wbp7 Controls Macrophage Function through GPI Glycolipid Anchor Synthesis. [ChIP_seq]
Histone methyltransferases catalyze site-specific deposition of methyl groups, enabling recruitment of transcriptional regulators. In mammals, trimethylation of lysine 4 in histone H3, a modification localized at the transcription start sites of active genes, is catalyzed by six enzymes (SET1a and S...
ORGANISM(S): Mus musculus 
2012-04-09 | E-GEOD-30972 | biostudies-arrayexpress
ProteomicsGPI glycan analysis of GPI-anchored protein from yeast
Quality controlled lipid-based protein sorting into selective ER exit sites
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2022-03-28 | PXD032852 | JPOST Repository
ProteomicsA novel structure of GPI anchored proteins
GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is atta...
ORGANISM(S): Homo sapiens (Human) 
2022-05-31 | PXD021996 | Pride
Human Pigg Gpi anchor Lc-esi- ms 
MetabolomicsThe putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast (Metabolome assay)
Cellular nutrient states control whether cells proliferate, or whether they enter or exit quiescence. Here, we report characterizations of fission yeast temperature-sensitive (ts) mutants of the evolutionarily conserved transmembrane protein, Cwh43, and explore its relevance to utilization of glucos...
2018-08-17 | MTBLS577 | MetaboLights
MetabolomicsThe putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast (Lipidome assay)
Cellular nutrient states control whether cells proliferate, or whether they enter or exit quiescence. Here, we report characterizations of fission yeast temperature-sensitive (ts) mutants of the evolutionarily conserved transmembrane protein, Cwh43, and explore its relevance to utilization of glucos...
2018-08-21 | MTBLS578 | MetaboLights
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