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TranscriptomicsTranscriptome profiling of e11.25 mouse pancreas: comparison between Ngn3-/- and Ngn3-/- ; Pdx1::Ngn3ER TM pancreatic epithelium
Individual pancreatic buds of crosses between Ngn3+/- and Ngn3+/- ; Pdx1::Ngn3ER TM -IRES-GFP were dissected. Pregnant females were injected intra-peritoneal with 2mg of Tamoxifen at e10.25 (9 am) and dissected at e11.25 (9 to 11 am). Most of the mesenchymal tissue was removed to reduce the tissue c...
ORGANISM(S): Mus musculus 
2014-12-02 | E-MTAB-3139 | biostudies-arrayexpress
TranscriptomicsEffect of Bicaudal C1 deletion on E13.5 mouse pancreatic mRNA profile
Bicaudal C1 KO embryonic pancreas develops cysts and has less endocrine progenitors after E14.5 (14.5 days after fertilization), while no defect is observed at E13.5. Bicaudal C1 is an RNA-binding protein. To understand the molecular mechanisms leading to both phenotypes, the mRNA expression profile...
ORGANISM(S): Mus musculus 
2015-04-07 | E-GEOD-58833 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of human pancreas organoids in expansion conditions in the presence of DMSO, CHIR99021 or CHIR99021 in the absence of FGF2
To investigate signaling pathways regulating human pancreas differentiation and morphogenesis, we developed a high-content image-based screen and quantitative multivariate analysis pipelines robust to heterogeneity to extract single-cell and organoid features using pancreatic progenitor organoids. A...
ORGANISM(S): Homo sapiens 
2026-01-21 | E-MTAB-16105 | biostudies-arrayexpress
GenomicsPtf1a binding sites in a pancreatic cell line which mimics embryonic day E10.5 pancreas progenitors.
Pancreatic specific transcription factor1a (Ptf1a) immunoprecipitated chromatin from 266.6 cells produced 26 million tags with the Illumina high throughput sequencing technology. Many of the mapped tags were genes which were found to be differentially expressed at E10.5 in the microarray experiments...
ORGANISM(S): Mus musculus 
2013-02-14 | E-GEOD-27295 | biostudies-arrayexpress
GenomicsMus musculus
Effect of Bicaudal C1 deletion on E13.5 mouse pancreatic mRNA profile
PRJNA253628 | ENA
TranscriptomicsComparative gene expression of mouse Pancreatic specific transcription factor 1a (Ptf1a/p48) in pancreatic progenitor cells
Ptf1a was identified as the essential transcription factor which controls pancreatic exocrine enzyme expression. With lineage tracing eperiments Ptf1a was recognized as an important pancreatic progenitor transcription factor and Ptf1a null mice do not develop a pancreas. We used gene expression arra...
ORGANISM(S): Mus musculus 
2011-01-24 | E-GEOD-26816 | biostudies-arrayexpress
TranscriptomicsBulk RNA sequencing of pancreatic progenitor spheroids treated with CHIR99021 and control (DMSO) conditions
H9 human embryonic stem cells with one allele of the PDX1 gene genetically modified into a reporter PDX1-P2A-H2B-GFP and one allele of NEUROG3 gene genetically modified into NEUROG3-P2A-tagRFP-T-NLS were differentiated into the pancreatic lineage until Stage 4 Day 3 of the in vitro differentiation p...
ORGANISM(S): Homo sapiens 
2025-11-14 | E-MTAB-14625 | biostudies-arrayexpress
TranscriptomicsCOUP-TFII Controls Mouse Postnatal Pancreatic β-Cell Mass through GLP-1 β-Catenin
Pancreatic β-cells have the key homeostatic function of releasing insulin to keep blood glucose in the normal range. It is not fully understood how β-cell mass is determined. Ablation of a nuclear receptor, chicken ovalbumin upstream promoter transcription factor II (COUP-TFII), in mouse pancreat...
ORGANISM(S): Rattus norvegicus 
2011-10-06 | E-GEOD-30526 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA sequencing of human embryonic stem cell-derived pancreatic cells enriched for early endocrine progenitors using a novel dual NEUROG3-reporter
Human embryonic stem cells with one allele of the NEUROG3 gene genetically modified into a fusion gene NEUROG3-LINKER-TAGRFPT-P2A-EGFP-NLS were differentiated into the pancreatic lineage until Stage 4 Day 1 of the in vitro differentiation protocol (Rezania et al., 2014, as modified in Petersen et al...
ORGANISM(S): Homo sapiens 
2022-12-31 | E-MTAB-11208 | biostudies-arrayexpress
TranscriptomicsBulk RNA sequencing of cycling and non-cycling NEUROG3-positive and negative human embryonic stem cell-derived pancreatic cells using a dual NEUROG3-reporter
Human embryonic stem cells with one allele of the NEUROG3 gene genetically modified into a fusion gene NEUROG3-LINKER-TAGRFPT-P2A-EGFP-NLS were differentiated into the pancreatic lineage until Stage 4 Day 1 of the in vitro differentiation protocol (Rezania et al., 2014, as modified in Petersen et al...
ORGANISM(S): Homo sapiens 
2022-12-31 | E-MTAB-11210 | biostudies-arrayexpress
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