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ProteomicsSpatial resolution of proteomic changes in lung tissue from a flexible AAV-DTR/DT mouse model of acute cell deletion mimicking epithelial lung injury
Lung epithelial injury leads to different pathologies, all associated with severe outcome and increased mortality. These pathologies are divided into chronic and acute diseases like idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD) or the acute lung distress syndrome ...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-30 | PXD025712 | Pride
Laser-capture microdissection Tmt Diphtheria toxin Ffpe Aav Dtr Epithelial cells 
ProteomicsLaser-capture proteomics of human FFPE substantia nigra
Laser-capture microdissection (LCM) allows the visualization and isolation of morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In combination with formalin-fixed and paraffin-embedded (FFPE) tissue it provides a powerful tool for retrospective and clinically rele...
ORGANISM(S): Homo sapiens (Human) 
2020-03-05 | PXD016563 | Pride
Laser-capture microdissection Tmt Ffpe Substantia nigra 
ProteomicsLung proteome of the AAV-DTR/DT mouse model of acute epithelial lung injury
Injury of the lung epithelium is one of the initial events driving acute lung diseases such as acute lung injury and acute respiratory distress syndrome. Repeated epithelial injury followed by aberrant repair leads to fibroblast activation and extracellular matrix deposition and is therefore conside...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-12-04 | MSV000088510 | MassIVE
ProteomicsProteome of senescent human lung fibroblasts and Panc-1 cells
Proteomic analysis of proliferating and senescent normal healthy lung fibroblasts (NHLFs) and Panc-1 cells. Senescence was induced using 5 uM Palbociclib for 6 days. Cells were lysed in SDS lysis buffer (2% SDS in 100 mM TEAB), boiled (15 min, 95 °C, 500 rpm) and sonicated in a sonication bath (10 ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-10-28 | MSV000090607 | MassIVE
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