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TranscriptomicsProgranulin A mediated MET signalling is essential for liver morphogenesis in zebrafish
Regulation of embryonic liver growth remains largely elusive. Progranulin has been discussed in pathological liver growth; however, the functional role of Pgrn in embryonic liver growth has never been addressed. Knockdown of GrnA, the orthologue of mammalian pgrn in zebrafish, displayed a deficien...
ORGANISM(S): Danio rerio 
2010-11-10 | E-GEOD-19211 | biostudies-arrayexpress
TranscriptomicsCas9 gRNA engineering for selectable genome editing, activation and repression
RNA-Seq after Cas9-gRNA transfection with different length gRNAs we performed PolyA Selection and RNA-Seq on cells transfected with dCas9-VPR and a gRNA of each length (20nt, 16nt, or 14nt) targeting ACTC1, MIAT, or HBG1/2
ORGANISM(S): Homo sapiens 
2015-07-15 | E-GEOD-70694 | biostudies-arrayexpress
GenomicsRNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs
RNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs
PRJNA588065 | ENA
GenomicsA genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the specificity of their target site recognition. Most previous studies have examined predicted off-target bi...
ORGANISM(S): Homo sapiens 
2015-03-04 | E-GEOD-61099 | biostudies-arrayexpress
TranscriptomicsZebrafish 22 hpf embryos: WT vs. MO-grnA injected
Transcriptional profiling of zebrafish embryos comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for the determination of expression profiling at 22 hpf under GRN-A deficiency. Two-condition experiment: wild type vs. MO-grnA treated cells. 3 b...
ORGANISM(S): Danio rerio 
2013-03-28 | E-GEOD-28318 | biostudies-arrayexpress
GenomicsGenome Editing with Tyrosinase gRNA
Genome Editing with Tyrosinase gRNA
PRJNA937212 | ENA
TranscriptomicsWT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos
Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for determination of expression profiling of trunk muscle at 16, 24, 48, 72 hpf under GRN-A deficiency. Two-condition experiment, wild type vs. MO-...
ORGANISM(S): Danio rerio 
2013-06-01 | E-GEOD-38441 | biostudies-arrayexpress
TranscriptomicsscRNA-seq CRISPR screening of human posterior brain organoids
We used a pooled, multiplexed CRISPR/Cas9 gene knock-out (KO) experiment with single-cell transcriptomic readout to perturb and validate selected TF regulomes . We designed gRNAs and generated a pooled lentiviral library targeting 12 TFs with specific midbrain or hindbrain expression or high regulat...
ORGANISM(S): Homo sapiens 
2026-03-17 | E-MTAB-15826 | biostudies-arrayexpress
TranscriptomicsCas9 target DNA specificity in vitro and in vivo (S. cerevisiae)
Cas9, a CRISPR RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although Cas9 offers remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the understanding of Cas9 target::homology requirements, w...
ORGANISM(S): synthetic construct 
2016-06-10 | E-GEOD-79667 | biostudies-arrayexpress
GenomicsIDLV capture for gRNA off-target analysis
IDLV capture for gRNA off-target analysis
PRJNA552824 | ENA
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