This dataset contains the LC-MS/MS data of three biological replicates of GTP-N-probe labeling without or with the presence of excess amount of GTP, and the LC-MS/MS data of GTP-N-probe labeling of recombinant GST-KRAS-G12C without or with the presence of GTP or AMG510.
Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., J Biol Chem 293(13): 4752-4766). Among them, we choose 12 kinase...
To define the signalling of extracellular GTP as enhancer of myogenesis, we investigated if the gene expression profile of differentiated C2C12 cells (4 hours in DM) was affected by extracellular GTP. Two-condition experiment, GTP-treated C2C12 cells vs Control C2C12 cells at 4 h of differentiation....
Smith2009 - RGS mediated GTP hydrolysis
This model is described in the article:
Dual positive and negative regulation of GPCR signaling by GTP hydrolysis.
Smith B, Hill C, Godfrey EL, Rand D, van den Berg H, Thornton S, H...
Physiological changes in GTP levels in live cells have never been considered a regulatory step of RAC1 activation because GTP concentrations determined by chromatography or mass spectrometry were shown to be substantially higher than the RAC1 GTP dissociation constant (RAC1-GTP Kd) determined in vit...
To define the signalling of extracellular GTP as enhancer of myogenesis, we investigated if the gene expression profile of differentiated C2C12 cells (4 and 24 hours in SM) was affected by extracellular GTP. Two-condition experiment, GTP-treated C2C12 cells vs Control C2C12 cells at 4 h and 24 h of ...
