To determined the phosphorylation sites of proteins after overproduction of PfkA in MPAO1 via pHERD20T-fpkA, and cells without overproduciton of this protein was used as a control.
To determined the effects of KK and KKP on the phosphorylation levels of proteins in PAO1, we overexpressed KK and KKP separately in PAO1 cells via pHERD20T based plasmids.
To determined the phosphorylation sites of proteins after overproduction of PfkA, PfkB and PfpC individually in PAO1 via pHERD20T-pfkA, pHERD20T-pfkB and pHERD20T-pfpC.
To determined the phosphorylation sites of MvaU purified from MPAO1, PAO1, PAO1/pHERD20T-fpkA, PAO1/pHERD20T-fpkB, PAO1/pHERD20T-fpkA-pfkB and PAO1/pHERD20T-fpkA-pfkB-pfpC
To determined the phosphorylation sites of proteins affected during the infection of T4 phage, we performed the experiments. Group13 is KKP overproduction, group 14 is vecto+T4, group 15 is KKP+T4
