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ProteomicsTMT-based proteomics analysis of GV, GVBD and MII oocytes
Two replicates of GV, GVBD, and MII oocytes were subjected to the 6-plex TMT labeling, HP-RP fractionation, and LC-MS/MS analysis. Two labeling experiments were performed for the total four replicates of each stage of oocytes.
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD018777 | Pride
Lc-msms Mii Oocytes Mouse Gvbd Gv 
ProteomicsQuantitative phosphoproteomics analysis of GV, GVBD and MII oocytes
Five replicates of GV, GVBD, and MII oocytes were subjected to the 15-plex TMT labeling, HP-RP fractionation, and LC-MS/MS analysis. For each replicate, 2,000 oocytes were collected from each of the GV, GVBD, and MII stages. Ti4+-IMAC was used to enrich phosphopeptides.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-13 | PXD050645 | Pride
Phosphoproteomics Mouse Lc-ms/ms Oocyte 
TranscriptomicsSAGE in Cultured Murine COC During Early Maturation
In cultured cumulus oocyte complexes (COC) FSH induces gene transcription required for germinal vesicle breakdown (GVBD). Experiments were performed to determine the critical period when gene transcription is required for GVBD and identify candidate mRNAs involved. Experiment I: murine COC were cult...
ORGANISM(S): Mus musculus 
2006-08-31 | E-GEOD-5568 | biostudies-arrayexpress
UnknownCould fertility clinics offer a sizable improvement of live birth rates by maturing post-GVBD oocytes in vitro?
Not available
S-EPMC6730726 | biostudies-literature
TranscriptomicsSAGE in Cultured Murine COC During Early Maturation
In cultured cumulus oocyte complexes (COC) FSH induces gene transcription required for germinal vesicle breakdown (GVBD). Experiments were performed to determine the critical period when gene transcription is required for GVBD and identify candidate mRNAs involved. Experiment I: murine COC were cult...
ORGANISM(S): Mus musculus 
2006-09-01 | GSE5568 | GEO
TranscriptomicsEXOSC10 sculpts the transcriptome during oocyte growth-to-maturation transition
Growing mammalian oocytes accumulate substantial amounts of RNA, most of which are degraded during the subsequent maturation stage. The growth-to-maturation transition begins with germinal vesicle breakdown (GVBD, envisioned as nuclear envelope breakdown) and is critical for oocyte quality. However,...
ORGANISM(S): Mus musculus 
2019-11-30 | GSE141190 | GEO
TranscriptomicsTranscriptional signature of progesterone-induced germinal vesicle breakdown in the fathead minnow ovary (Pimephales promelas)
A growing number of studies have examined transcriptional responses to sex steroids along the hypothalamic-pituitary-gonadal axis in teleost fishes. However, data is are lacking on the molecular signaling cascades that underlie progesterone signaling. The objectives of the present study were to ch...
ORGANISM(S): Pimephales promelas 
2013-07-19 | E-GEOD-43601 | biostudies-arrayexpress
TranscriptomicsTranscriptional signature of progesterone-induced germinal vesicle breakdown in the fathead minnow ovary (Pimephales promelas)
A growing number of studies have examined transcriptional responses to sex steroids along the hypothalamic-pituitary-gonadal axis in teleost fishes. However, data is are lacking on the molecular signaling cascades that underlie progesterone signaling. The objectives of the present study were to char...
ORGANISM(S): Pimephales promelas 
2013-07-19 | GSE43601 | GEO
UnknownChronic restraint stress disturbs meiotic resumption through APC/C-mediated cyclin B1 excessive degradation in mouse oocytes.
Not available
S-EPMC6133342 | biostudies-literature
UnknownRole of inhibin and activin in the modulation of gonadotropin- and steroid-induced oocyte maturation in the teleost Fundulus heteroclitus.
Not available
S-EPMC1891301 | biostudies-literature
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