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ProteomicsProteins of anti-sigma70 and anti-RNAP immunoprecipitates in bacteria
As part of the project aimed at the interaction of RNA with RNA polymerase and primary sigma factor in bacteria we analyzed anti-σ70 antibody immunoprecipitates from Mycobacterium smegmatis stationary phase to identify proteins in σA complex in non-treated cells. We performed mass spectrometry analy...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Corynebacterium glutamicum ATCC 13032 
2024-03-06 | PXD047705 | Pride
Sigma a Rnap Transcription Corynebacterium glutamicum Mycobacterium smegmatis 
ProteomicsAntibody Characterisation using FFAP
Modern therapeutic approaches, especially for cancer and certain autoimmune disorders, evolved and have started to frequently rely on monoclonal antibodies (mAb) or other recombinantly prepared biomolecules (biopharmaceuticals) instead of on small molecules. The mAb treatment is effective but comes ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD044326 | Pride
Covalent labeling Protein structure Mass spectrometry Ffap Togni agents 
ProteomicsTop-down detection of oxidative protein footprinting by Collision-Induced Dissociation, Electron-Transfer Dissociation and Electron-Capture Dissociation
Fast photochemical oxidation of proteins (FPOP) footprinting is a structural mass spectrometry method that maps proteins by fast and irreversible chemical reactions. The position of oxidative modification reflects solvent accessibility and site reactivity and thus provides information about protein ...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2023-07-20 | PXD031926 | Pride
Electron-transfer dissociation Fast photochemical oxidation of proteins Top-down Collision-induced dissociation Mass spectrometry. Electron-capture dissociation 
ProteomicsStructural aspects of FOXO4 binding to DAF16 investigated with FPOP using both bottom-up and top-down MS.
A combination of covalent labelling techniques and mass spectrometry (MS) is currently a progressive approach for the de-riving insights relating to the mapping of protein surfaces or protein-ligand interactions. In this study, we mapped an interac-tion interface between DNA binding domain (DBD) of ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-14 | PXD027624 | Pride
Bottom-up proteomics Dna Transcription factors Protein Foxo4 Fast photochemical oxidation of proteins Top-dow proteomics 
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