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TranscriptomicsDatabase for mRNA Half-Life of 19,977 Genes Obtained by DNA Microarray Analysis of Pluripotent and Differentiating Mouse
Degradation of mRNA is one of the key processes that control the steady-state level of gene expression. However, the rate of mRNA decay for the majority of genes is not known. We successfully obtained the rate of mRNA decay for 19 977 non-redundant genes by microarray analysis of RNA samples obtaine...
ORGANISM(S): Mus musculus 
2010-05-16 | E-GEOD-13609 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human B-cells and mouse NIH-3T3 cells for analysis of RNA synthesis, abundance and decay
This SuperSeries is composed of the following subset Series:; GSE9973: Half-life determination for human B-cells (BL41); GSE9975: newly transcribed RNA (nt-RNA) for IFN alpha and gamma time course; GSE9977: Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma fo...
ORGANISM(S): Homo sapiens 
2009-10-10 | E-GEOD-10026 | biostudies-arrayexpress
TranscriptomicsHalf-life determination for human B-cells (BL41)
RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100µM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis. Only probe sets providing present calls in all RNA samples/subsets were included into the analysis We used ...
ORGANISM(S): Homo sapiens 
2009-09-30 | GSE9973 | GEO
TranscriptomicsRNA half-life analysis after transcriptional blocking with actinomycin D
Transcriptional profiling of HeLa cells treated with actinomycin D for increasing amounts of time. Relative abundance to control cells was used to estimate the half-life of lncRNAs. Two-condition experiment, actinomycin D-treated vs. untreatted HeLa cells. Biological replicates: 2. Technical replic...
ORGANISM(S): Homo sapiens 
2015-06-30 | E-GEOD-62963 | biostudies-arrayexpress
TranscriptomicsExpression data from RNA half-life experiments in Prochlorococcus
RNA decay was measured in Prochlorococcus after inhibition of transcription by rifampicin using customized Affymetrix gene expression arrays. RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investiga...
ORGANISM(S): Prochlorococcus marinus subsp. pastoris str. CCMP1986 
2010-05-16 | E-GEOD-17075 | biostudies-arrayexpress
GenomicsHomo sapiens
Half-life determination for human B-cells (BL41)
PRJNA109051 | ENA
ProteomicsAn Approach to Measuring Protein Turnover in Human Induced Pluripotent Stem Cell Organoids by Mass Spectrometry
Patient-derived organoids from induced pluripotent stem cells have emerged as a model for studying human diseases beyond conventional two-dimensional (2D) cell culture. As such, the development of robust methods to study global proteostasis and protein turnover in organoids will remain essential as ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-16 | PXD032169 | Pride
Midbrain organoid Midbrain Silac Half-life determination Turnover D3-leucine Topograph Organoid 
UnknownFirst direct determination of the 93Mo half-life.
Not available
S-EPMC8492754 | biostudies-literature
TranscriptomicsExpression data from NIH-3T3 cells used for half-life determination
Data from tc-, nt- and p-RNA as well as 1 and 2h of actinomycin-D treatment (5µg/ml) of NIH-3T3 cells used to determine half-lives. RNA was labeled for 15, 30 or 60 minutes with 4-thiouridine. After preparation of tc-RNA, thiol-labeled RNA was biotinylated using biot-HPDP and subsequently tc-RNA was...
ORGANISM(S): Mus musculus 
2009-09-30 | GSE10011 | GEO
TranscriptomicsChanges in mRNA Stability Associated with Cold Stress in Arabidopsis Cells
Control of mRNA half-life is a powerful strategy to adjust individual mRNA levels to various stress conditions, because the mRNA degradation rate controls not only the steady-state mRNA level but also the transition speed of mRNA levels. Here, we analyzed mRNA half-life changes in response to cold s...
ORGANISM(S): Arabidopsis thaliana 
2012-12-16 | E-GEOD-31837 | biostudies-arrayexpress
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