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TranscriptomicsSmall RNAs in Chlamydomonas reinhardtii
RNA populations in Chlamydomonas reinhardtii Keywords: Highly parallel pyrosequencing Small RNAs were prepared from Chlamydomonas reinhardtii total extracts,ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of 454 cloning pr...
ORGANISM(S): Chlamydomonas reinhardtii 
2007-05-15 | E-GEOD-7308 | biostudies-arrayexpress
TranscriptomicsCharacterization of active piRNAs and piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 (RNA-seq)
We have found that oncogenic Ras combined with loss of the Hippo tumour-suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells, coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from generative loci, which are normally restr...
ORGANISM(S): Drosophila melanogaster 
2016-08-01 | E-GEOD-83843 | biostudies-arrayexpress
TranscriptomicsCharacterization of active piRNAs and an piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 (small RNA)
We have found that oncogenic Ras combined with loss of the Hippo tumour-suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells, coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from generative loci, which are normally restr...
ORGANISM(S): Drosophila melanogaster 
2016-08-01 | E-GEOD-83871 | biostudies-arrayexpress
ProteomicsSpecialization of the Drosophila nuclear export family protein, Nxf3, for piRNA precursor export
The piRNA pathway is a conserved small RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon mobilisation. In Drosophila ovaries, most piRNAs originate from dual-strand clusters, which generate piRNAs from both genomic strands. Dual-strand clusters use...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-07-29 | PXD014472 | Pride
Pirna rna export piwi drosophila transposons 
ProteomicsDaedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-04-18 | PXD013404 | Pride
Pirna piwi drosophila 
ProteomicsDaedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-04-18 | PXD013405 | Pride
Pirna piwi drosophila 
ProteomicsDaedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-04-18 | PXD013403 | Pride
Pirna piwi drosophila 
ProteomicsDaedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-04-18 | PXD013417 | Pride
Pirna piwi drosophila 
ProteomicsRequirement of Nuclear Pore Complex Subunits for transposon silencing in Drosophila
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. In Drosophila ovaries, piRNAs are produced from discrete genomic loci, called piRNA clusters, which are composed of inactive transposon copies and fragments and thus constitute a genetical...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-04-15 | PXD019670 | Pride
Pirna piwi npc nuclear export 
ProteomicsRequirement of Nuclear Pore Complex subunits for transposon silencing in Drosophila
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. In Drosophila ovaries, piRNAs are produced from discrete genomic loci, called piRNA clusters, which are composed of inactive transposon copies and fragments and thus constitute a genetical...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-04-16 | PXD019671 | Pride
Pirna piwi npc nuclear export 
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