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TranscriptomicsTruncation of LOC100288798 (SLC38A4-AS) lncRNA in human haploid KBM7 cell line
Many thousand long non-coding (lnc) RNAs are mapped in the human genome. Time consuming studies using reverse genetic approaches by post-transcriptional knock-down or genetic modification of the locus demonstrated diverse biological functions for a few of these transcripts. The Human Gene Trap Mutan...
ORGANISM(S): Homo sapiens 
2015-12-22 | E-GEOD-71284 | biostudies-arrayexpress
TranscriptomicsmRNA expression data from AG-haESC, E14 and MEF
Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes.Here,we report the derivation of haESCs from androgenetic blastocysts. These cells, which we designated AG-haESCs, express classical ESC markers, are pluripotent, and contribute to various tissues includ...
ORGANISM(S): Mus musculus 
2012-04-26 | E-GEOD-35785 | biostudies-arrayexpress
GenomicsNGS based viability screening using haploid cell line
Identification of human deubiquitylating enzymes whose knock out result in hypersensitivity to DNA damaging agents, by comparing the sequence reads of ‘barcode region’ from mixed cell culture.
EGAS00001001095 | EGA
TranscriptomicsRetroviral mutagenesis in haploid ES cells
Retroviral integration sites were mapped using inverse PCR to assess preferred integration sites of this virus in the genome after infection with the retrovirus and its impact on gene expression. Inverse PCR was applied on genomic DNA to identify retroviral integration sites. Inverse PCR allows to i...
ORGANISM(S): Mus musculus 
2011-10-25 | E-GEOD-33184 | biostudies-arrayexpress
GenomicsHaploid genome-wide screens for essential components and negative regulators of interferon lambda signaling in human cells
We undertook a unbiased genome-wide haploid genetic screen to identify new components in interferon lambda signaling. In addition, we performed a genome-wide screen to identify genes that repress spontaneous activation of interferon stimulated genes in the absence of interferon. Both of these screen...
ORGANISM(S): Homo sapiens 
2017-07-25 | E-MTAB-5866 | biostudies-arrayexpress
GenomicsIdentifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
To search for factors regulating paternally imprinted genes (PEGs), we performed a genome-wide loss-of-function CRISPR/Cas9 screen in haploid parthenogenetic ESCs. This by staining a pooled CRISPR library with a PEG10 antibody and next FACS-sorted for cells that presented de-novo PEG10 expression.
ORGANISM(S): Homo sapiens 
2021-10-21 | E-MTAB-11014 | biostudies-arrayexpress
ProteomicsMissing proteins identified from the haploid cell lines: HAP1 and KBM-7
Chromosome-centric Human Proteomic Project (C-HPP) aims at identifying and characterizing protein products encoded from all protein-coding genes of human chromosomes. As evident in the recent nature papers, it is possible that those missing proteins may be low abundant in many cell types or expresse...
ORGANISM(S): Homo sapiens (Human) 
2018-10-01 | PXD006614 | Pride
Lc-msms Missing proteins Kbm-7 Haploid cell line Hap1 
ProteomicsMissing proteins identified from the haploid cell lines: HAP1 and KBM-7
Chromosome-centric Human Proteomic Project (C-HPP) aims at identifying and characterizing protein products encoded from all protein-coding genes of human chromosomes. As evident in the recent nature papers, it is possible that those missing proteins may be low abundant in many cell types or expresse...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-10 | MSV000083952 | MassIVE
TranscriptomicsRNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells
We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). Ubiquitin specific peptidase 14 (USP14) was a significant hit. In order to validate USP14 as a regulator of ISG expression, we created knockouts of USP14 in HAP1 cells usi...
ORGANISM(S): Homo sapiens 
2017-07-25 | E-MTAB-5865 | biostudies-arrayexpress
TranscriptomicsRNA-seq of coding RNA from DDX6 knockout human haploid (HAP1) cells
We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). DEAD-box helicase 6 (DDX6) was a significant hit. In order to validate DDX6 as a regulator of ISG expression, we created knockouts of DDX6 in HAP1 cells using CRISPR-Cas9 ...
ORGANISM(S): Homo sapiens 
2017-07-25 | E-MTAB-5867 | biostudies-arrayexpress
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