HEK293T cells were transiently transfected with plasmids encoding Maltose binding protein (MBP)-tagged proteolytically inactive CAPN (CAPN3:CS) and constitutively active form of Fyn (Fyn-YF). CAPN3:CS was pulldown from the HEK293T cell lysates using amylose-resin, purified as an excised band from SD...
ORGANISM(S): Homo Sapiens (human)