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ProteomicsChanges to SUMO-modified proteome and total cellular proteome during EBV ractivation in AGS gastric carcinoma cells
To monitor changes to cellular and viral SUMO-modified proteins during EBV reactivation. SUMO1 and SUMO2 conjugates were studied using KGG mutant forms of SUMO and GG-K specific antibody enrichment of SUMO modified peptides. Time-points of 12h and 24h post-reactivation were monitored. Total cellular...
ORGANISM(S): Homo sapiens (Human) 
2023-06-19 | PXD034631 | Pride
Sumo epstein-barr virus ebvhuman herpes virus 4 hhv4 
ProteomicsAntibody RING-Mediated Destruction (ARMeD) of endogenous proteins
To understand the cellular function of a gene the encoding DNA or mRNA transcript can be manipulated and the consequences observed. However, these approaches do not have a direct effect on the protein product of the gene, which is either permanently abrogated or depleted at a rate defined by the hal...
ORGANISM(S): Homo sapiens (Human) 
2020-05-29 | PXD018113 | Pride
Ring ubiquitin nanobody endogenous degradation 
ProteomicsTotal cellular proteome analysis of mutant and WT HNF4alpha Hepatic Progenitor Stage Cells
Post-translational modification of proteins has been shown to control different aspects of protein biology. We have previously implicated a SUMO consensus motif in HNF4alpha’s carboxylic terminus as an important regulator of protein biology during stem cell differentiation. In this study, we have ge...
ORGANISM(S): Homo sapiens (Human) 
2019-06-17 | PXD013737 | Pride
Human Hnf4alpha Embryonic stem cells Hepatic progenitor Sumo 
ProteomicsPhotocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions
Almost all cellular processes are influenced by ubiquitination. A large family of enzymes known as E3 ligases provide the specificity for ubiquitination, with the largest class among them, the RING E3s, comprising over 600 members in humans. RING E3s facilitate transfer of ubiquitin to substrates by...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075613 | Pride
Ubiquitin n-maleimido-diazirine e3 ligase activity-based probe 
ProteomicsSites of aspirin-mediated lysine acetylation in HeLa cells
Aspirin, or acetylsalicylic acid is widely used to control pain, inflammation and fever. Important to this function is its ability to irreversibly acetylate cyclooxygenases at active site serines. Aspirin has the potential to acetylate other amino-acid side-chains, leading to speculation that aspiri...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080778 | MassIVE
ProteomicsSites of aspirin-mediated lysine acetylation in HeLa cells, part 2
Aspirin, or acetylsalicylic acid is widely used to control pain, inflammation and fever. Important to this function is its ability to irreversibly acetylate cyclooxygenases at active site serines. Aspirin has the potential to acetylate other amino-acid side-chains, leading to speculation that aspiri...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080852 | MassIVE
ProteomicsProteome-wide identification of SUMO2 modification sites
Posttranslational modification with small ubiquitin-like modifiers (SUMOs) alters the function of proteins involved in diverse cellular processes. SUMOs are conjugated to lysine residues in target proteins by SUMO-specific enzymes. Although proteomic studies have identified hundreds of sumoylated su...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080741 | MassIVE
ProteomicsProteome-wide identification of SUMO2 modification sites
Posttranslational modification with small ubiquitin-like modifiers (SUMOs) alters the function of proteins involved in diverse cellular processes. SUMOs are conjugated to lysine residues in target proteins by SUMO-specific enzymes. Although proteomic studies have identified hundreds of sumoylated su...
ORGANISM(S): Homo sapiens (Human) 
2014-10-15 | PXD001281 | Pride
Heat-shock Sumo modification sites Hek293 
ProteomicsA SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis
During Caenorhabditis elegans oocyte meiosis, a multi-protein complex localised between homologous chromosomes, the ring complex (RC), promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure...
ORGANISM(S): Caenorhabditis elegans 
2020-10-15 | PXD005202 | Pride
Klp-19 Pias C. elegans Sumoylation sites 
ProteomicsIdentification of SUMO targets required to maintain human stem cells in the pluripotent state. - Identification of SUMO modified proteins from hiPS cells
To determine the role of SUMO modification in the maintenance of pluripotent stem cells we used ML792, a potent and selective inhibitor of SUMO Activating Enzyme. Treatment of human induced pluripotent stem cells with ML792 initiated changes associated with loss of pluripotency such as reduced expre...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD028050 | Pride
Sumo Hipsc Stem cells 
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