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ProteomicsCharacterization of COVID-19 survivors 6-month after discharge
we applied quantitative proteomic and metabolomic approach to analyze plasma samples from health subjects and COVID-19 survivors six months after discharge
ORGANISM(S): Homo Sapiens 
2022-01-19 | PXD025148 |
ProteomicsImprovement of Peptide Separation for Exploring the Missing Proteins Localized on Membranes
How to separate hydrophobic peptides and to prevent loss of such peptides, nevertheless, have not been drawn enough attention (19). As a membrane protein possesses higher hydrophobicity, especially for one with multiple bilayer-exposed domains, efficient detection of hydrophobic peptides becomes a c...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD010630 | Pride
Missing proteins High concentration organic solvent Membrane fraction Lc ms/ms 
ProteomicsHuman HeLa cells LC MS/MSLC-MSMS
We developed a reagent of 10-plex isobaric tags (IBT) with high stability and low cost. The labeled peptides were sensitively detected on Orbitrap Q Exactive MS with MS2 resolution of 35000 at 30% NCE, while the peptides were efficiently labelled over 97% by IBT at ratio of 20:1 of reagent/peptide (...
ORGANISM(S): Homo sapiens (Human) 
2022-03-04 | PXD008681 | Pride
Hela cells Lc-msms 
ProteomicsHuman bladder,colon,kidney,liver cancer LC MS/MS
We propose that enrichment of low-abundance proteins benefits MPs finding. ProteoMinerTM is an equalizing technique by reducing high-abundance proteins and enriching low-abundance proteins in biological liquids. With triton X-100/TBS buffer extraction, ProteoMinerTM enrichment and peptide fractionat...
ORGANISM(S): Homo sapiens (Human) 
2017-10-02 | PXD006833 | Pride
Kidney Liver Colon Lc ms/ms" "bladder 
ProteomicsConotoxin Exploitation from Conus betulinus
Employing Conus betulinus as a representative, we sequenced and assembled the first Conus genome. After integration of multi-omics data, we provide novel insights into the genetic central dogma of conotoxins, assuming a number ratio of ~1:1:10s for genes/transcripts/peptides.
ORGANISM(S): conus betulinus 
2021-09-08 | PXD014892 | Pride
Conus  genome  conotoxins  multi-omics  transcript  proteomics  peptide 
ProteomicsAnalysis of the Byssus of Chinese Green Mussel proteomics by LC-MS/MS
Here, we integrated high-throughput transcriptome and proteome sequencing to construct a comprehensive protein database for the byssus of Chinese green mussel (Perna viridis), aiming at providing novel insights into the molecular mechanisms of byssal binding to heavy metals.
ORGANISM(S): Perna viridis 
2019-04-30 | PXD009183 | Pride
Proteomics Byssus Lc-msms 
ProteomicsHuman placental tissue LC-MS/MS
This project aimed at missing protein identification from human placental tissue, combining sample preparation and mass spectrometry technology development.For sample preparation, low molecular proteins were enriched by C18 solid phase extraction, SDS-PAGE and MWCO filter. For LC-MS/MS analysis, mic...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-29 | MSV000086385 | MassIVE
ProteomicsHuman bladder,colon,kidney,liver cancer LC MS/MS
We propose that enrichment of low-abundance proteins benefits MPs finding. ProteoMinerTM is an equalizing technique by reducing high-abundance proteins and enriching low-abundance proteins in biological liquids. With triton X-100/TBS buffer extraction, ProteoMinerTM enrichment and peptide fractionat...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-25 | MSV000081649 | MassIVE
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