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ProteomicsPlant UDP-N-acetylglucosamine (UDP-GlcNAc) transporter (UGNT1) glycopeptide analysis
Glycosylation reactions require activated glycosyl donors in form of nucleotide sugars to drive processes such as post-translational protein modifications, glycolipid and polysaccharide biosynthesis. Most of these reactions occur in the Golgi requiring cytosolic-derived nucleotide sugars, which are ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-09-24 | PXD006635 | Pride
Udp-n-acetylglucosamine Glycopeptide Nucleotide sugar transporters 
ProteomicsProfiling the level of N-glycan structural heterogeneity in Arabidopsis N-glycoproteins
In this study, we use hydrophilic enrichment, high-resolution tandem mass spectrometry with complimentary and triggered fragmentation to profile Arabidopsis N-glycopeptides. A total of 492 N-glycosites were identified from 324 Arabidopsis proteins with extensive N-glycan structural heterogeneity rev...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-12-18 | PXD006270 | Pride
Arabidopsis Glycopeptides N-glycosylation 
ProteomicsA novel tool, Multiple Marker Abundance Profiling, uses Selected Reaction Monitoring and data-dependent acquisition for rapid estimation of organelle abundance in subcellular samples.
Methods were investigated that would deliver rapid and semi-quantitative analysis of the subcellular composition of Arabidopsis samples, as the standard technique for this i.e. immunoblotting is difficult to quantify and relies on a very few protein targets, whose responses to developmental or envir...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD004576 | Pride
Srm Subcellular proteomics Organelle Arabidopsis Quantitation Shotgun 
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