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ProteomicsProteomic Profiling of HEK293-F cell and human peripheral blood neutrophil proteins extracted by conventional direct extraction and phenol-guanidinium isothiocyanate (P/GTC)-based extraction methods
To evaluate the performance of the phenol-guanidinium isothiocyanate (P/GTC)-based extraction method in proteomic analysis, we performed proteome analysis from HEK293-F cell and human peripheral blood neutrophil proteins extracted by the P/GTC-based extraction and conventional direct extraction meth...
ORGANISM(S): Homo Sapiens (human) 
2019-10-28 | PXD011487 | JPOST Repository
GenomicsPHF3 binds RNA polymerase II via the SPOC domain and regulates transcription of neuronal genes - TFIIS, H3K27me3, F-12 ChIP-seq
ChIP-seq of TFIIS, H3K27me3, and F-12 in WT, KO, and dSPOC HEK293 cells. ChIP-seq of PHF3 - GFP in WT HEK293 cells
ORGANISM(S): Homo sapiens 
2021-09-07 | E-MTAB-8789 | biostudies-arrayexpress
TranscriptomicsFusobacterium nucleatum upregulates the immune inhibitory receptor PD-L1 in intestinal epithelial cells via the activation of ALPK1
Fusobacterium nucleatum is a Gram-negative oncobacterium that is associated with colorectal cancer. The molecular mechanisms utilized by F. nucleatum to promote colorectal tumor development have largely focused on adhesin-mediated binding to the tumor tissue and on the pro-inflammatory capacity of F...
ORGANISM(S): Homo sapiens 
2024-05-13 | GSE267317 | GEO
ProteomicsHuman cell lines pulldown interactions CL-MSMS
Using a combination of GFP-trap affinity purification, native gel band identification and APEX2-mediated BioID pulse-chase analysis, we investigate an import pathway and folding mechanism for histones H3 and H4. We investigated proteins associating with H3 and H4 bearing mutations along alpha-helix ...
ORGANISM(S): Homo sapiens (Human) 
2022-09-15 | PXD029354 | Pride
Human Hek293-f Lc-msms Hela frt Warwick_rtp Histone chaperone Bioid 
GenomicsPHF3 binds RNA polymerase II via the SPOC domain and regulates transcription of neuronal genes - TFIIS, H3K27me3, F-12 ChIP-seq
PHF3 binds RNA polymerase II via the SPOC domain and regulates transcription of neuronal genes - TFIIS, H3K27me3, F-12 ChIP-seq
PRJEB36735 | ENA
UnknownNovel blockers of hyperpolarization-activated current with isoform selectivity in recombinant cells and native tissue.
Not available
S-EPMC3417492 | biostudies-literature
UnknownValidation of a commercial antibody to detect endogenous human nicastrin by immunoblot.
Not available
S-EPMC7194342 | biostudies-literature
UnknownDifferent non-synonymous polymorphisms modulate the interaction of the WRN protein to its protein partners and its enzymatic activities.
Not available
S-EPMC5349866 | biostudies-literature
UnknownUnsymmetrically Substituted Dibenzo[b,f][1,5]-diazocine-6,12(5H,11H)dione-A Convenient Scaffold for Bioactive Molecule Design.
Not available
S-EPMC7070320 | biostudies-literature
TranscriptomicsTranscription profiling of human HEK 293 cells with or without overexpression of the repressor ICER treated by forskolin at 0,1,2,4 and 8 hours
HEK 293 cells with or without overexpression of ICER IIg were treated with forskolin for 0, 1 h, 2 h, 4 h or 8 h. The cell line used in the experiments is HEK 293 cells stable transfected with tetracycline-inducible ICER (Inducible cAMP early repressor) expression.
ORGANISM(S): Homo sapiens 
2010-01-01 | E-MTAB-122 | biostudies-arrayexpress
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