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TranscriptomicsTranscription profiling by high throughput sequencing of HeLa human cancer cell line
Illumina single-end sequencing of Hela_2 (HeLa cell line). While the number and identity of proteins expressed in a single human cell type is currently unknown, this fundamental question can be addressed by advanced mass spectrometry (MS)-based proteomics. On-line liquid chromatography coupled to hi...
ORGANISM(S): Homo sapiens 
2011-11-12 | E-MTAB-823 | biostudies-arrayexpress
ProteomicsConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-12 | MSV000081607 | MassIVE
TranscriptomicsHeLa Adenovirus timecourse
A timecourse of adenovirus infection of human HeLa cells, using RNA-seq
ORGANISM(S): Homo sapiens 
2012-10-27 | E-MTAB-1277 | biostudies-arrayexpress
TranscriptomicsAnalysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
UnknownExploring the HeLa Dark Mitochondrial Proteome.
Not available
S-EPMC7066081 | biostudies-literature
ProteomicsExploring the HeLa Dark Mitochondrial proteome
In the framework of the Human Proteome Project initiative, we aim to improve mapping and characterization of mitochondrial proteome. In this work we implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochon...
ORGANISM(S): Homo sapiens (Human) 
2020-02-27 | PXD014200 | Pride
Personalised medicine Subproteome Mass spectrometry Mitochondria Drug targets Dark proteome 
ProteomicsOff-target responses in the HeLa proteome subsequent to transient plasmid transfection using SILAC
We report a significant differential expression of proteins, all of which are associated with a viral infection response typified by Interferon type I/II. Notably the upregulated proteins may affect several vital cellular processes, including cell cycle control, translation and post-translational m...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-05-23 | MSV000079757 | MassIVE
ProteomicsiTRAQ-based quantitative proteomic analysis of nuclear proteome in VPS35-depleted HeLa cells
Stable VPS35 knockdown HeLa cells were generated using lentiviral short hairpin RNA (shRNA) construct. Isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry was used to measure the changes of nuclear protein abundance in VPS35-depleted HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD027166 | Pride
Itraq  vps35  nuclear proteome  hela cells 
ProteomicsConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset...
ORGANISM(S): Homo sapiens (Human) 
2014-04-16 | PXD000900 | Pride
Lc-ms/ms Digestion Enzymes Non-tryptic 
ProteomicsExploring the HeLa Dark Mitochondrial proteome
In the framework of the Human Proteome Project initiative, we aim to improve mapping and characterization of mitochondrial proteome. In this work we implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochon...
ORGANISM(S): Homo sapiens (Human) 
2020-02-27 | PXD014201 | Pride
Personalised medicine Human mitochondria Dark proteins 
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