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ProteomicsTransposon sequencing uncovers an essential regulatory function of phosphoribulokinase for methylotrophy
Methylotrophy is the ability of organisms to grow at the expense of reduced one-carbon compounds such as methanol or methane. Here, we used transposon sequencing combining hyper-saturated transposon mutagenesis with high-throughput sequencing to define the essential methylotrophy genome of Methyloba...
ORGANISM(S): Methylobacterium extorquens PA1 
2017-07-11 | PXD006834 | Pride
Methylobacterium extorquens pa1 Lc-ms/ms Label-free quantification 
ProteomicsUse of rare-earth elements in the phyllosphere colonizer Methylobacterium extorquens PA1
Until recently, rare-earth elements (REEs) had been thought to be biologically inactive. This view changed with the discovery of the methanol dehydrogenase (Mdh) XoxF that strictly relies on REEs for its activity. Some methylotrophs only contain xoxF, while others, including the model phyllosphere c...
ORGANISM(S): Methylobacterium extorquens PA1 
2019-01-21 | PXD011842 | Pride
Rare earth metals Arabidopsis Methanol Ion transport Proteomics Methylobacterium extorquens 
ProteomicsDynamic character displacement among a pair of bacterial phyllosphere commensals in situ
Differences between species promote stable coexistence in a resource-limited environment. These differences can result from interspecies competition leading to character shifts, a process referred to as character displacement. While character displacement is often interpreted as a consequence of gen...
ORGANISM(S): Rhizobium sp. Leaf68 Sphingomonas sp. Leaf257 
2022-05-26 | PXD026619 | Pride
Sphingomonas Phyllosphere Dynamic character displacement Rhizobium Proteomics 
ProteomicsGeneration of an Escherichia coli strain growing on methanol as sole energy and carbon source via the ribulose monophosphate cycle
The increasing demand for non-food competitive carbon sources such as methanol for biotechnology has brought methanol-utilizing bacteria, so-called methylotrophs, to focus. The product spectrum of natural methylotrophs and their genetic accessibility is limited and as an alternative approach, the in...
ORGANISM(S): Escherichia coli 
2022-08-05 | PXD034138 | Pride
Synthetic methylotrophy Proteomics E.coli 
MetabolomicsA general non-self response as part of plant immunity
Plants, like other multicellular lifeforms, are colonized by microorganisms. How plants respond to their microbiota is currently not well understood. We used a phylogenetically diverse set of 39 endogenous bacterial strains from Arabidopsis thaliana leaves to assess host transcriptional and metaboli...
2021-09-13 | MTBLS2522 | MetaboLights
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