Sort   by:  
 Page size 
ProteomicsUpdated in-gel digestion for protein analysis by mass spectrometry
The in-gel digestion of proteins for analysis by liquid chromatograph mass spectrometry has been used since the early 1990s. Although several improvements have contributed to increasing the quality of the data obtained, many recent publications still use sub-optimal approaches. We present an updated...
ORGANISM(S): Homo sapiens (Human) 
2018-10-02 | PXD009600 | Pride
Proteomics Mass spectrometry In-gel digestion Trypsin 
ProteomicsEvidence for the nuclear import of monomeric histones H3.1 & H4
We present here evidence that histones H3.1 and H4 can be imported into the nucleus as monomers in human cells. Using a tether-and-release system to study the cytosolic phase and import dynamics of newly synthesised histones, we find that H3.1 and H4 can be maintained as stable monomers in the cytos...
ORGANISM(S): Homo sapiens (Human) 
2018-09-10 | PXD009915 | Pride
Human Histones 
ProteomicsIdentification of an RNA sponge that controls the levels, processing and efficacy of the RoxS riboregulator of central metabolism in B. subtilis
Only a few small regulatory RNAs (sRNAs) have been characterized in B. subtilis, the paradigm of Gram-positive bacteria, and one of the major challenges is target identification. Here we use global in vivo RNA psoralen cross-linking to identify RNA-RNA partners in Bacillus subtilis. Two sRNAs, RoxS ...
ORGANISM(S): Bacteria Bacillus subtilis 
2021-09-08 | PXD015051 | Pride
Metabolism Roxs Rosa Bacillus subtilis Rnas 
Sort   by:  
 Page size