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ProteomicsComparative integrated omics analysis of the Hfq regulon in Bordetella pertussis
This study represents the first attempt to characterize the RNA chaperone Hfq regulon in bacterial pathogen using an integrative omics approach. Gene expression profiles were analyzed by RNA-seq and protein amounts in cell-associated and cell-free fractions were determined by LC-MS/MS technique. Com...
ORGANISM(S): Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) 
2019-07-02 | PXD013953 | Pride
Hfq regulon Lfq ms Bordetella pertussis 
TranscriptomicsComparison of the expression profiles of 630E strain and a hfQ mutant after 7.5h of growth in Tyt
Clostridium difficile is an emergent human pathogen and the most common cause of nosocomial diarrhea. Our recent deep-sequencing data strongly suggest the importance of RNA-based mechanisms for the control of gene expression in C. difficile. The RNA chaperon protein Hfq is a key component of post-tr...
ORGANISM(S): Peptoclostridium difficile 
2014-07-15 | E-GEOD-49168 | biostudies-arrayexpress
TranscriptomicsKlebsiella pneumoniae: WT vs. Hfq mutant
Transcriptional profiling of K. pneumoniae comparing the wild type strain (S5) with a hfq deletion mutant (H0201). Goal was to determine the effects of Hfq absence on global K. pneumoniae gene expression. WT vs. Hfq mutant. Eight replicates for each ORF presented in the 44K array.
ORGANISM(S): Klebsiella pneumoniae 
2013-01-11 | E-GEOD-29448 | biostudies-arrayexpress
ProteomicsAn integrative, multi-omics analysis of the Hfq regulon
The ability of bacteria to respond to environmental change is based on the exploration of sophisticated regulatory landscapes. While we can learn a great deal from reductive analyses of individual pathways, and global approaches to gene regulation, a deeper understanding of these complex signalling ...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) 
2017-10-18 | PXD005621 | Pride
Hfq Pseudomonas fluorescens Posttranscriptional regulation Multi-omics Proteomics Isobaric labelling 
TranscriptomicsComparison of the expression profiles of 630E strain and an Hfq antisense after 7.5h of growth in Tyt
Transcriptional profiling of C. difficile 630E strain vs. an Hfq antisense after 7.5h of growth inTYt. Two-conditions experiments, 630E strain vs. Hfq antisense grown in TYt, 4 biological replicates for each condition.
ORGANISM(S): Peptoclostridium difficile 
2014-07-15 | E-GEOD-55273 | biostudies-arrayexpress
GenomicsAnalysis of the Hfq regulon in Pseudomonas fluorescens SBW25.
Analysis of the Hfq regulon in Pseudomonas fluorescens SBW25.
PRJEB19015 | ENA
TranscriptomicsIdentification of RNA molecules associated with the RNA-chaperone Hfq
We used high-throughput methods to discover the Salmonella RNAs that are targeted by the bacterial Sm-like protein, Hfq. Our generic approach, using chromosomal epitope tagging and coIP-on-chip will also be useful to identify the post-transcriptional regulons of many other RNA-binding proteins. Key...
ORGANISM(S): Salmonella enterica 
2010-06-20 | E-GEOD-10149 | biostudies-arrayexpress
TranscriptomicsIdentification of Hfq bound RNA by co-immunoprecipitation and RNA-sequencing
Co-immunoprecipitation with endogenous Hfq3xFlag in exponential, stationary, non-induced and virulence induced conditions, followed by RNA-sequencing, revealed 1697 mRNAs and 208 ncRNAs associated with Hfq. We identified 56 new ncRNAs and validated 3 Hfq-dependent trans sRNAs on by Northern blot ana...
ORGANISM(S): Agrobacterium tumefaciens 
2014-12-04 | E-GEOD-59123 | biostudies-arrayexpress
UnknownComparative Integrated Omics Analysis of the Hfq Regulon in Bordetella pertussis.
Not available
S-EPMC6627887 | biostudies-literature
UnknownThe Hfq regulon of Neisseria meningitidis.
Not available
S-EPMC5458458 | biostudies-literature
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