Sort   by:  
 Page size 
ProteomicsInvestigating the conformational changes of OEA on HIF-3A-ARNT by HDX-MS
We used hydrogen-deuterium exchange mass spectrometry (HDX-MS) to investigate the influence of OEA on HIF-3A-ARNT.HDX-MS data indicated that OEA binding stabilized multiple residues within the Gβ and Fα regions of HIF-3A PAS-B domain.It is possible that the stabilized Gβ and Fα function as an “inter...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-20 | PXD033376 | Pride
Oea Hdx-ms Hif-3a-arnt 
TranscriptomicsHIF-1 dependent gene expression upon S. aureus infection
HIF-1 is an important transcription factor for immune responses to bacterial infection. We wanted to analyze the HIF-1 dependent gene expression upon S. aureus infection and analyzed the gene expression of HepG2 nt and HepG2 HIF-1-/- cells four hours upon infection using affymetrix human gene 1.0 st...
ORGANISM(S): Homo sapiens 
2013-08-01 | E-GEOD-44943 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human Burkittas lymphoma cell line, P493-6 to identify hypoxia responsive genes
Activation of glycolytic genes by HIF-1 is considered critical for metabolic adaptation to hypoxia. We found that HIF-1 also actively suppresses glucose metabolism through the tricarboxylic acid cycle (TCA) by directly trans-activating the gene encoding pyruvate dehydrogenase kinase 1 (PDK1). PDK1...
ORGANISM(S): Homo sapiens 
2008-06-12 | E-GEOD-4086 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryonic fibroblasts from embryos with ?CH1 mutation in p300 and/or CBP atreated with hypoxia or dipyridyl (a hypoxia mimetic) and trichostatin A (a histone deacetylase inhibitor) reveals two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression
The C-terminal activation domain (C-TAD) of the hypoxia-inducible transcription factors HIF-1? and HIF-2? binds the CH1 domains of the related transcriptional coactivators CREB-binding protein (CBP) and p300, an oxygen-regulated interaction thought to be highly essential for hypoxia-responsive trans...
ORGANISM(S): Mus musculus 
2008-06-12 | E-GEOD-3318 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of C. elegans hypoxia responses
The goal of the study was to identify hypoxia-induced gene expression changes in C. elegans and to determine which of these responses to hypoxia were regulated by hif-1. Towards these aims, we analyzed mRNA expression patterns in synchronized populations of wild-type worms that were cultured in stan...
ORGANISM(S): Caenorhabditis elegans 
2005-06-20 | E-SMDB-3266 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse prostate from AKT transgenic or wild type littermate controls treated with RAD001 (MTOR inhibition) or placebo reveals mTOR inhibition reverses Akt-dependent prostate intraepithelial neoplasia through regulation of apoptotic and HIF-1-dependent pathways reveals
Transgenic (Probasin driven Myr-AKT)or wild-type littermates were treated with RAD001 or placebo and sacrificed at 12 and 48 hours following the beginning of treatment
ORGANISM(S): Mus musculus 
2007-09-29 | E-GEOD-1413 | biostudies-arrayexpress
TranscriptomicsThe role of HIF-1 in beta-glucan induced response in myeloid cell
beta-glucan induced glycolysis in HIF-1 depedent manner. We reported that beta-glucan injection in mice led to upregulated glycolysis. HIF-1a plays a major role in this process. Mice receives beta-glucan via ip for 4 days. Splenocytes were isolated for RNA sequencing.
ORGANISM(S): Mus musculus 
2014-09-26 | E-GEOD-57206 | biostudies-arrayexpress
TranscriptomicsMicroarray-assisted Cloning of Mutants: Expression Profiling of tom-1 and unc-43
Analysis of gene expression data in two C.elegans mutant strains: KP3293 tom-1(nu468) and KP3365 unc-43(n1186); hif-1(nu469). These results support the utility of microarray hybridizations to facilitate positional cloning.
ORGANISM(S): Caenorhabditis elegans 
2007-06-27 | E-GEOD-2210 | biostudies-arrayexpress
TranscriptomicsGenome-wide mapping of Hif-1α binding sites in zebrafish [microarray]
Analysis of gene expression changes in the von Hippel Lindau when mutant compared to wild-type at 4dpf using a whole genome microarray expression profiling. The von Hippel Lindau mutant displays a systemic hypoxic response under normoxic conditions. We performed single-colour microarrays to identify...
ORGANISM(S): Danio rerio 
2016-03-22 | E-GEOD-70885 | biostudies-arrayexpress
TranscriptomicsExpression profiling of hypoxic HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells: time course
Analysis of expression changes of cultured HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells subjected to hypoxia (0.5% O2) for 0, 4, 8, 12 hours . Results provide insight to cell type-specific response to hypoxia. HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells were collec...
ORGANISM(S): Homo sapiens 
2010-06-21 | E-GEOD-18494 | biostudies-arrayexpress
Sort   by:  
 Page size